Vet Med - Czech, 2006, 51(3):89-92 | DOI: 10.17221/5525-VETMED

Appearance of iron-labeled blood mononuclear cells in electron microscopy

D. Horky1, I. Lauschova1, M. Klabusay2, M. Doubek2, P. Sheer3, S. Palsa2, J. Doubek3
1 Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
2 Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic
3 Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic

Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.

Keywords: ultrastructure; iron nanoparticles; cultivation; animal model

Published: March 31, 2006  Show citation

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Horky D, Lauschova I, Klabusay M, Doubek M, Sheer P, Palsa S, Doubek J. Appearance of iron-labeled blood mononuclear cells in electron microscopy. Vet Med - Czech. 2006;51(3):89-92. doi: 10.17221/5525-VETMED.
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