Veterinární medicína, 2006 (vol. 51), issue 9
The role of neutrophil apoptosis during experimentally induced Streptococcus uberismastitis
Z. Sladek, D. Rysanek, H. Ryznarova, M. Faldyna
Vet Med - Czech, 2006, 51(9):437-447 | DOI: 10.17221/5571-VETMED 
The object of the study was to determine if apoptosis of neutrophils and their subsequent elimination from the mammary gland by macrophages are modulated by an infection of Streptococcus uberis. The experiments were carried out in 5 clinically normal Holstein × Bohemian Red Pied crossbred heifers, aged 14 to 18 months. Before the experimental infection mammary glands were stimulated by PBS as a control. The samples of cell populations were obtained by lavages of the mammary glands in 4 intervals (24, 48, 72 and 168 h) after the PBS and after the experimental infection. Flow cytometry was used to determine the Annexin V positive and propidium...
Iodine content in raw milk
J. Travnicek, I. Herzig, J. Kursa, V. Kroupova, M. Navratilova
Vet Med - Czech, 2006, 51(9):448-453 | DOI: 10.17221/5574-VETMED
The paper presents the latest information on iodine content in raw milk, in the CzechRepublic. In 2005, iodine was determined using the Sandell-Kolthoff method in 169 milk samples, taken from transportation tanks (capacity 11 000-13 000 litres) of collecting milk for dairy processing, from 14 areas of South-western Bohemia. The average iodine content in milk samples was 442.5 ± 185.6 µg/l, minimum and maximum value were 68.6 and 1 000.6 µg/l respectively. 81.7% of the samples contained more than 250 µg/l. The average iodine content in milk from the collecting areas ranged from 230.2 ± 133.0 µg/l to 702.7 ±...
The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos
M. Lopatarova, S. Cech, L. Holy, R. Dolezel
Vet Med - Czech, 2006, 51(9):454-460 | DOI: 10.17221/5576-VETMED
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a control (Group C). Bovine embryos (Day6.5-7.5) collected from superovulated cows were classified according to developmental stages and morphological qualities (Grade 1 and 2) before cryopreservation and they were transferred to synchronized heifers after thawing. Pregnancy rates after transfer of morulae, early blastocysts and expanded blastocysts in Group V compared to Group C (54.5%, 12/22 vs. 56.0%, 14/25; 53.3%, 16/30 vs. 58.1%,...
Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003
L. Czanderlova, P. Hlozek, D. Chmelar, P. Lany
Vet Med - Czech, 2006, 51(9):461-467 | DOI: 10.17221/5578-VETMED
Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isolates prepared by anaerobic cultivation was carried out by multiplex PCR. C. perfringens type A (37.9%) and type C (1.3%) were detected on 58 and 2 farms, respectively. The cpb2 gene was found in 79.3% strains of C. perfringens type A and in 100% strains of type C. An evaluation of the prevalence of positive identification of C. perfringens with the cpb2 gene shows that the microorganism was...