Veterinární medicína, 2007 (vol. 52), issue 7
Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review
Z. Hubalkova, P. Kralik, B. Tremlova, E. Rencova
Vet Med - Czech, 2007, 52(7):273-292 | DOI: 10.17221/2044-VETMED 
The purpose of the present study was to give an overview of imported and traded gadoid fish species (Gadiformes) in the Czech Republic and to describe available methods for their authentication. Due to the increasing interest of customers in the purchase of buy fish meat and other seafood animals, it is necessary to have available analytical methods with discriminating power of respective fish species. With regard to different values and prices of various fish species, these may be adultered. Until recently, electrophoretic, chromatographic and immunological methods based on the analysis of proteins extracted from fish musculature seemed to...
Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig
M. Zalecki, J. Calka, M. Lakomy
Vet Med - Czech, 2007, 52(7):293-300 | DOI: 10.17221/2041-VETMED
We explored the immunoreactivity of choline acetyltransferase (ChAT) in the cranial nerve motor nuclei of the porcine rhombencephalon to reveal the cholinergic nature of these regions. In our experiments we used an immunohistochemical method for the visualization of all acetylcholine-containing structures. All studied motor nuclei contained ChAT-positive cell bodies and fibres, but the intensity of staining differed between the nuclei. Furthermore, characteristic ChAT-immunoreactive bouton-like structures, which are known to be synaptic terminals of the cholinergic system, were observed in the borders of all studied regions. The localization of ChAT-positive...
Presence and characteristics of sorbitol-negative Escherichia coli O157 in healthy sheep faeces
H. Turutoglu, D. Ozturk, L. Guler, F. Pehlivanoglu
Vet Med - Czech, 2007, 52(7):301-307 | DOI: 10.17221/2042-VETMED
The presence of sorbitol-negative Escherichia coli O157 was investigated in healthy Awassi sheep faeces from 175 randomly selected animals in Burdur province of Turkey. Out of 175 animals, 16 (9.1%) were faecal shedding of sorbitol-negative E. coli O157. Out of the 15 flocks included in the study, 7 (47%) had at least one sheep positive for sorbitol-negative E. coli O157. The isolation rate of sorbitol-negative E. coli O157 ranged from 8.3 to 60% among the animals tested in the flocks. A total of 16 ovine sorbitol-negative E. coli O157 strains were characterized by a multiplex PCR. Results showed that 6 (37.7%) strains...
Detection of spring viraemia of carp virus (SVCV) with monoclonal antibodies
S. Reschova, D. Pokorova, Z. Nevorankova, J. Hulova, T. Vesely
Vet Med - Czech, 2007, 52(7):308-316 | DOI: 10.17221/2043-VETMED
Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV), a severe disease in cyprinid fish, were prepared. Nine MAbs were characterised using Western blotting (WB) where all reacted with glycoprotein G, except for MAb 2E1, which showed no reactivity in WB. All nine MAbs showed specificity in an immunoperoxidase test. In ELISA assays, their titres ranged between 1:32 000 and 1:128 000. A panel of SVCV isolates from different European regions were set up and examined by sandwich ELISA assay using the MAbs at a concentration of 15 μg/ml. Only MAb 4C12/3C8 showed low sensitivity in most of the isolates at an absorbance of A450...