Veterinární medicína, 2008 (vol. 53), issue 4
Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows
P. Jawor, S. Steiner, T. Stefaniak, W. Baumgartner, A. Rzasa
Vet Med - Czech, 2008, 53(4):173-183 | DOI: 10.17221/1920-VETMED 
The purpose of this study was to assess the diagnostic value of fibrinogen, haptoglobin, and serum amyloid A determination in the monitoring of the treatment of limb diseases in dairy cows. Fourteen lame cows were examined, while 10 clinically healthy cows constituted the control group. Blood samples from the ill animals were collected on three occasions: (1) upon arrival at the clinic, (2) between the third and sixth day after arriving, and (3) upon return to the owner. Blood samples from the control cows were collected once. Plasma levels of fibrinogen, haptoglobin, serum amyloid A, and total serum protein and its fractions (albumin, α-, β-,...
Bacterial D-alanine concentrations as a marker of bacterial nitrogen in the gastrointestinal tract of pigs and cows
U. Schoenhusen, J. Voigt, U. Hennig, S. Kuhla, R. Zitnan, W.-B. Souffrant
Vet Med - Czech, 2008, 53(4):184-192 | DOI: 10.17221/1922-VETMED
D-alanine (DAL) has been successfully used as a marker of bacterial nitrogen (N) in the small intestine of cows. This study compares DAL contents of intestinal bacteria in digesta of cows and pigs with respect to diet and sampling site. In isolated ileal bacteria of pigs a DAL/N ratio (41.72 ± 3.19 mg/g, n = 18) was found, which was not different from that in rumen bacteria (40.11 ± 1.95 mg/g, n = 18) but higher than in duodenal bacteria of cows (38.09 ± 2.09 mg/g, n = 18, P < 0.001). The DAL/N ratio in ileal bacteria of pigs was independent of the diet (P = 0.38) but it tended to be affected...
Prevalence of gastrospirillum-like organisms in pigs, cattle, and dogs: a comparison of diagnostic methods between species
L. Kolodzieyski, B. Kim, H. Park, H.S. Yoon, C.W. Lim
Vet Med - Czech, 2008, 53(4):193-202 | DOI: 10.17221/1919-VETMED
A survey was conducted to determine the prevalence of spiral-shaped bacteria in animals as a possible source of pathogens causing chronic changes in the human and animal stomach as well as in other parts of the digestive tract. This study was carried out in three different groups of animals, pigs, cattle and dogs. Swabs from the oral cavity of dogs (n = 198) were stained using Gram's method to evaluate gastrospirillum-like organisms (GLOs) and revealed two different types of GLOs with an incidence of 23.2% (46/198). The stomachs of the pigs (n = 104), cattle (n = 102), and dogs (n = 7) were collected for the urease test,...
Apparent digestibility in meat-type guinea pigs as determined by total collection or by internal marker
P. Guevara, T. Claeys, G.P.J. Janssens
Vet Med - Czech, 2008, 53(4):203-206 | DOI: 10.17221/1917-VETMED
Six adult male meat-type guinea pigs were subjected to three subsequent digestibility trials with 100% alfalfa, 50% alfalfa and 50% sugar cane and 50% alfalfa and 50% concentrate. For each animal in each period, feed intakes were recorded and all faeces were collected and stored at -20°C until analysis. Feeds and faeces were analysed for dry matter, crude ash, crude protein, ether-extract, crude fibre and acid-insoluble ash. Coefficients of apparent digestibility were calculated according to both the total collection method and the marker method with acid-insoluble ash as internal marker. The present study suggests that acid-insoluble ash is not valid...
Immunoglobulin producing cells in chickens immunized with Eimeria tenella gametocyte antigen vaccines
M.M. Ayaz, M. Akhtar, I. Hussain, F. Muhammad, A.U. Haq
Vet Med - Czech, 2008, 53(4):207-213 | DOI: 10.17221/1918-VETMED
The present paper reports on the IgA, IgG and IgM antibodies secreting cells (ASC) in the spleen of chickens vaccinated with E. tenella (local isolates) gametocyte vaccine(s) by using Enzyme Linked Immunospot (ELISPOT) assay. Irrespective of the vaccine used, the number of IgG antibody secreting cells (ASC) in the spleen of orally vaccinated chickens was higher than the number of IgA and IgM ASC. Maximum numbers of IgG, IgA and IgM ASC were found in chickens vaccinated with sonicated gametocyte formalin inactivated vaccine (Group III) followed by formalin inactivated gametocytes (Group II) and gametocytes (Group I). The number of ASC (IgG, IgA...
Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis
H.B. Lee, M.R. Alam, J.W. Seol, N.S. Kim
Vet Med - Czech, 2008, 53(4):214-220 | DOI: 10.17221/1921-VETMED
The aim of this study was to determine if the activity of tartrate-resistant acid phosphatase (TRAP) in the synovial fluid (SF) and serum can be used as a marker for diagnosing the early stages of osteoarthritis (OA). We also wished to determine if identifiable differences in the concentrations of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) could be detected in SF between normal joints and OA joints for the diagnosis of early OA. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and medial meniscectomy. Five sham-operated beagle dogs were...
Ileal adenocarcinoma in a cow: a case report
M. Floeck, S. Hoegler, R. Krametter-Froetscher
Vet Med - Czech, 2008, 53(4):221-223 | DOI: 10.17221/1924-VETMED
An 11-year-old Pinzgauer cow was presented with signs of traumatic reticuloperitonitis. Sonographical examination revealed ascites, peritonitis and a dilated amotile small intestine, which was confirmed during laparotomy. On the basis of necropsy and pathohistological findings a diagnosis of metastasizing ileal adenocarcinoma was made.
Reconstruction of the oesophagus using pedicle diaphragm and omentum flaps in a dog: a case report
H.B. Lee, M.R. Alam, N.S. Kim
Vet Med - Czech, 2008, 53(4):224-228 | DOI: 10.17221/1923-VETMED
A two-years-old, intact female, Shih-Tzu dog weighing 5 kg was presented to the Chonbuk Animal Medical Centre, College of Veterinary Medicine, Chonbuk National University, with the history of bone ingestion before two days. The survey radiographs of the thorax revealed a bone at the caudal thoracic oesophagus. An oesophagram was performed which did not reveal any oesophageal leakage or perforation. The left caudolateral thoracotomy was performed and the caudal oesophagus was found partially necrosed, friable and inflamed. The necrosed, friable part was removed and the oesophageal defect was reconstructed using a full-thickness muscle flap collected...