Veterinární medicína, 2008 (vol. 53), issue 7
Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide
Z. Sladek, D. Rysanek
Vet Med - Czech, 2008, 53(7):347-357 | DOI: 10.17221/1991-VETMED
The aim of this study was to determine whether expression of CD14 on macrophages is regulated differently during initiation and resolution of the inflammatory response caused by CD14-dependent (lipopolysaccharide) and CD14-independent (muramyldipeptide) bacterial signals. In cell suspensions from the site of inflammation we observed two types of macrophages: non-vacuolized (NMAC) and vacuolized (VMAC) cells. NMAC (monocyte-like cells) were dominant during the early stage of the inflammatory response, whilst VMAC contained phagocytosed apoptotic neutrophils in various stages of digestion. These latter cells...
In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study
M. Machatkova, P. Hulinska, Z. Reckova, K. Hanzalova, J. Spanihelova, R. Pospisil
Vet Med - Czech, 2008, 53(7):358-364 | DOI: 10.17221/1993-VETMED
In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and...
Permanent jugular catheterization in miniature pig: treatment, clinical and pathological observatio
D. Usvald, J. Hlucilova, J. Strnadel, R. Prochazka, J. Motlik, M. Marsala
Vet Med - Czech, 2008, 53(7):365-372 | DOI: 10.17221/1992-VETMED
The aim of present study was the installation of permanent jugular catheter to miniature pigs, which are frequently used as animal model for the experiments closely related to human medicine. We describe here in many details surgical interventions leading to the localization and fixation of Seldinger needle in ν. jugularis externa and its use for extended period of time. Eight animals were included in these experiments and their heath status was currently monitored and no visible problems were recorded. After two months they were euthanased and potential function of catheters was carefully inspected. Only in two instances we found pathological...
The influence of baical skullcap root (Scutellaria baicalensis radix) in the diet of broiler chickens on the chemical composition of the muscles, selected performance traits of the animals and the sensory characteristics of the meat
B. Kroliczewska, W. Zawadzki, T. Skiba, W. Kopec, J. Kroliczewski
Vet Med - Czech, 2008, 53(7):373-380 | DOI: 10.17221/1994-VETMED
The aim of this study was to evaluate the effect of the baical skullcap root (Scutellaria baicalensis radix) on the performance, chemical composition of the muscles and sensory characteristics of the meat of broiler chickens. 120 one-day old Hubbard Hi-Y broiler hybrids were assigned to four groups of 30 each and fed either a basal diet (control) or a basal diet supplemented with 0.5%, 1.0% or 1.5% ground root of skullcap. The body weight, weight gain, feed consumption and feed : gain ratio were calculated after 42 days. The addition of the ground baical skullcap root to the fodder had no effect on the quality or chemical composition of the...
Intravenous endothelin-1 triggers pulmonary hypertension syndrome (ascites) in broilers
D.H. Zhou, J. Wu, S.J. Yang, D.C. Cheng, D.Z. Guo
Vet Med - Czech, 2008, 53(7):381-391 | DOI: 10.17221/1989-VETMED
Two experiments were conducted to evaluate the effect of endothelin-1(ET-1) on pulmonary arterial pressure (PAP) and pulmonary hypertension syndrome (PHS, ascites) morbidity in broilers. Two hundred and seventy of one-day-old Arbor Acre commercial broiler chickens were randomly allocated into two experiments. In Experiment 1, 40 broilers (28 days of age) were intravenously with five different dose groups of ET-1, and the PAP was measured from time 0 to 30 min. The results indicated that the PAP increased from time 0.5 to 5 min after the broilers were injected with ET-1 at concentrations of 24 ng/kg (Group T1), 120 ng/kg (Group T2), and 240 ng/kg (Group...
Heinz body anaemia in two dogs after Catalan spring onion ("calcot") ingestion: a case report
R. Guitart, C. Mateu, A. Lopez i Agullo, J. Alberola
Vet Med - Czech, 2008, 53(7):392-395 | DOI: 10.17221/1990-VETMED
Catalan spring onions, or "calcots", are a very popular kind of vegetables obtained by special agronomic practices that avoid photosynthesis and subsequent metabolism by the Allium cepa plants. They have been considered for more than a century harmless for pets, as pleasant odour and sweet taste differ so largely from any other onion that can be ingested by children and adults in huge quantities, up to ~3% body weight, without problems being observed. However, the organosulphur chemicals responsible for oxidative damage to canine red blood cells found in this plant species seem to remain present, as two dogs were confirmed to be poisoned after...