Veterinární medicína, 2008 (vol. 53), issue 9
Lactoferrin: a review
L. Adlerova, A. Bartoskova, M. Faldyna
Vet Med - Czech, 2008, 53(9):457-468 | DOI: 10.17221/1978-VETMED 
This review discusses the biological properties of the glycoprotein lactoferrin. Lactoferrin has been identified in secretions from exocrine glands and in specific granules of neutrophils. After degranulation, neutrophils become the main source of lactoferrin in blood plasma. Lactoferrin possesses various biological functions, including roles in iron metabolism, cell proliferation and differentiation, and antibacterial, antiviral, and antiparasitic activity. Many of these functions do not appear to be connected with its iron binding ability. Of late, lactoferrin concentrations have been measured mostly in humans but also in some other species. However,...
Nitrofuran antibiotics: a review on the application, prohibition and residual analysis
M. Vass, K. Hruska, M. Franek
Vet Med - Czech, 2008, 53(9):469-500 | DOI: 10.17221/1979-VETMED
Nitrofuran antibiotics, employed for the treatment of bacterial diseases in livestock production, were banned from use in the European Union (EU) in 1995 due to concerns about the carcinogenicity of their residues in edible tissue. This review provides an overview of nitrofuran toxicity, metabolism, and also specific aspects of legislation surrounding their prohibition. Special attention is devoted to semicarbazide - a nitrofuran metabolite and food contaminant. Analytical procedures for nitrofuran analysis in various matrices and validation requirements for screening and confirmation methods with respect to EU regulations are also reviewed.
Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture
H.B. Zhu, D.Z. Guo, S.J. Yang, Y.H. Zhang, H. Wang, H.T. Guo, Y. Zhang, D.C. Cheng
Vet Med - Czech, 2008, 53(9):501-509 | DOI: 10.17221/1981-VETMED
The osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we evaluated the effects of OGP on the growth and differentiation of bovine marrow mesenchymal stem cells in culture. Our results showed that OGP promoted osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of Osteocalcin (BGP)....
Distribution and transmission of Mycobacterium avium subspecies paratuberculosis in farmed red deer (Cervus elaphus) studied by faecal culture, serology and IS900 RFLP examinations
M. Kopecna, I. Parmova, L. Dvorska-Bartosova, M. Moravkova, V. Babak, I. Pavlik
Vet Med - Czech, 2008, 53(9):510-523 | DOI: 10.17221/1980-VETMED
The objectives of this study were the determination of Mycobacterium avium subsp. paratuberculosis (MAP) distribution in organs of farmed red deer (Cervus elaphus) and the investigation of its vertical and horizontal spread among animals, using serology, cultivation and the standardized IS900 RFLP method. During the three year of study, the production of antibodies for MAP increased from 0 in the first year to 7.7% (positive) and 0 to 88.5% (dubious) in the third year of the study. The first performed global culture examination of faecal samples from 28 animals was negative for MAP. In the three subsequent...