Veterinární medicína, 2009 (vol. 54), issue 9
Genotyping of bovine viral diarrhoea virus isolates from the Czech Republic
B. Robesova, K. Kovarcik, S. Vilcek
Vet Med - Czech, 2009, 54(9):393-398 | DOI: 10.17221/3053-VETMED 
This study was focused on the genetic typing of bovine viral diarrhoea virus (BVDV) isolates obtained from 41 serum samples of persistently infected cattle in the Czech Republic in the period of 2004 to 2007. For the differentiation of BVDV isolates, the 5'-UTR and Npro regions were selected. A 288-bp fragment from 5'-UTR and 428-bp fragment from Npro of the selected isolates were amplified by RT-PCR and subsequently sequenced and analysed by computer-assisted phylogenetic analysis. The isolates belong to BVDV-1 genotype and the following subtypes were identified: b (n = 16), d (n = 16), e (n = 2) and f (n = 7). In...
Clinical and ultrasonographic differences between cattle and buffaloes with various sequelae of traumatic reticuloperitonitis
A.M. Abdelaal, M. Floeck, S. El Maghawry, W. Baumgartner
Vet Med - Czech, 2009, 54(9):399-406 | DOI: 10.17221/128/2009-VETMED
This study was performed to document the clinical and ultrasonographic differences between cattle and buffaloes with various sequelae of traumatic reticuloperitonitis (TRP) and the importance of ultrasonography in detection of such sequelae. Twenty nine cows and 33 buffaloes with TRP were investigated. By using ultrasonography and some confirmatory techniques as paracentesis, laparotomy and necropsy, the various sequelae of TRP in both species were clarified. Acute local peritonitis, chronic local peritonitis, acute diffuse peritonitis, reticular abscesses, thoracic abscesses and pericarditis have been detected as sequelae of TRP in both cattle and...
The effects of partial replacement of corn silage on biochemical blood parameters in lactating primiparous dairy cows
U. Polat, H. Gencoglu, I.I. Turkmen
Vet Med - Czech, 2009, 54(9):407-411 | DOI: 10.17221/72/2009-VETMED
The aim of this experiment was to investigate the effects of partial replacement of corn silage with long alfalfa hay and/or coarse chopped wheat straw on biochemical parameters in late lactating Holstein primiparous dairy cows. Twelve late lactating Holstein primiparous cows including four cows equipped with a rumen cannula, averaging 210 ± 20 days in milk and weighing 575 ± 50 kg were randomly assigned in a 4 × 4 Latin square design. During each of four 21-days periods, cows were fed four total mixed diets that were varied in the forage sources: (1) 50% corn silage (CS), (2) 35% corn silage + 15% wheat straw (CSW), (3) 35% corn silage...
Altered plasma triglyceride-rich lipoproteins and triglyceride secretion in feed-restricted pregnant ewes
A. Mazur, M. Ozgo, Y. Rayssiguier
Vet Med - Czech, 2009, 54(9):412-418 | DOI: 10.17221/77/2009-VETMED
In late pregnancy, energy deficits in ewes can induce a metabolic imbalance, which often results in pregnancy toxaemia. This metabolic disorder is characterised by hypoglycaemia, hyperketonaemia, an increase in plasma concentrations of free fatty acids and by fatty infiltration in the liver. The purpose of the present study was to identify alterations in lipid and triglyceride-rich lipoprotein distribution and to evaluate triglyceride secretion in ewes during the third trimester of pregnancy, particularly in ewes with feed restriction. The study was performed on non-pregnant and on twin- and triplet-bearing pregnant Limousine × Romanov ewes fed a control...
Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm
Z. Stastkova, S. Karpiskova, R. Karpiskova
Vet Med - Czech, 2009, 54(9):419-426 | DOI: 10.17221/88/2009-VETMED
The aim of this study was to report the detection of methicillin-resistant Staphylococcus aureus (MRSA) strains at a veterinary university goat breeding farm and their characteristics. A total of 278 samples collected from animals, milk, environment and farm personnel between June 2006 and March 2008 were examined. The identification of S. aureus isolates was performed by a species specific PCR assay. All detected isolates were tested for resistance to oxacillin and other antimicrobials by phenotypic methods and for the mecA gene by PCR method. Eight MRSA were detected in this study. Five of them originated from goat's milk and...
The distribution and accumulation of phthalates in the organs and tissues of chicks after the administration of feedstuffs with different phthalate concentrations
A. Jarosova, J. Harazim, P. Suchy, L. Kratka, V. Stancova
Vet Med - Czech, 2009, 54(9):427-434 | DOI: 10.17221/2/2009-VETMED
For the monitoring of distribution and accumulation of phthalic acid esters (PAE) in animal tissues, samples of muscle, mesenteric fat (fat), skin and liver from broiler chicks ROSS 308 were used. The chicks were divided into four groups (50 chicks each). All the chicks were given commercial diets (complete feed, KKS) for broiler chicks (starter - BR1; grower - BR2 and finisher - BR3). The experimental diets were supplemented with vegetable oil (RV) with low (group N) or high (group V) phthalate contents, or animal fat with a high phthalate content (group Z). Neither the control diets (K), nor the grower (BR1) diets contained vegetable oils or animal...
Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR
K. Rosenbergova, P. Lany, Z. Pospisil, O. Kubicek, V. Celer, D. Molinkova
Vet Med - Czech, 2009, 54(9):435-443 | DOI: 10.17221/16/2009-VETMED
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic. The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe was developed for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. Conserved regions in the matrix protein gene of avian influenza virus served as targets for the primers and TaqMan probe design. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative...
Endoscopic findings, microbiological and cytological evaluation of tracheal aspirates in a population of Standardbred horses with poor performances
F. Laus, A.R. Attili, M. Cerquetella, A. Spaterna, B. Tesei, V. Cuteri
Vet Med - Czech, 2009, 54(9):444-450 | DOI: 10.17221/3/2009-VETMED
Sixty-two Standardbred horses housed at the same racetrack, with history of reduced exercise tolerance, cough lasting for at least two weeks and/or prolonged recovery time were clinically examined. An endoscopic examination of the nasopharynx, larynx and trachea to the level of the carina was performed, amount of mucus in trachea was registered and samples of tracheal wash for cytological and microbiological examinations was collected. A strong statistical association between amount of mucus in trachea and neutrophils percentage in tracheal wash was found. Bacteria isolated included Streptococcus equi subsp. zooepidemicus (14 horses),...
Concurrent lymphosarcoma and Salmonella enteritidis infection in a cat: a case report
M.I. Bhaiyat, H. Hariharan, A. Chikweto, E. Brathwaite-Sylvester, P.J.A. Burnett, V. Matthew, S. Oliveira, C. Johnson
Vet Med - Czech, 2009, 54(9):451-454 | DOI: 10.17221/64/2009-VETMED
Concurrent lymphosarcoma and salmonellosis in a 12-year-old female neutered domestic shorthair cat in Grenada is described. Clinically, the cat was emaciated, and had a history of vomiting and diarrhea of two months duration. Clinical examination revealed a large palpable mass in the craniodorsal abdomen and fluid in the thoracic cavity. Gross lesions consisted of moderate pyothorax and pyoabdomen with variably-sized, single to multiple, soft, white masses in the mesentery adjacent to the pancreas, on the serosal surface of the stomach, and on the quadrate lobe of the liver. Histopathological findings associated with these masses were compatible with...