Vet Med - Czech, 2009, 54(9):419-426 | DOI: 10.17221/88/2009-VETMED

Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

Z. Stastkova1, S. Karpiskova3, R. Karpiskova1,2
1 University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 National Institute of Public Health, Prague, Brno, Czech Republic
3 Czech Collection of Microorganisms, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic

The aim of this study was to report the detection of methicillin-resistant Staphylococcus aureus (MRSA) strains at a veterinary university goat breeding farm and their characteristics. A total of 278 samples collected from animals, milk, environment and farm personnel between June 2006 and March 2008 were examined. The identification of S. aureus isolates was performed by a species specific PCR assay. All detected isolates were tested for resistance to oxacillin and other antimicrobials by phenotypic methods and for the mecA gene by PCR method. Eight MRSA were detected in this study. Five of them originated from goat's milk and three were recovered from one human carrier of the farm personnel. All obtained MRSA isolates were clonally consistent and were characterized as: SCCmec type IV, spa type t064, seb positive and for genes encoding TSST-1, PVL and exfoliative toxins A and B negative.

Keywords: MRSA; PCR; mecA; Panton-Valentine leukocidin; toxic-shock syndrome toxin-1, staphylococcal enterotoxins; exfoliative toxins; SCCmec; PFGE; spa type

Published: September 30, 2009  Show citation

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Stastkova Z, Karpiskova S, Karpiskova R. Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm. Vet Med - Czech. 2009;54(9):419-426. doi: 10.17221/88/2009-VETMED.
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