Veterinární medicína, 2015 (vol. 60), issue 3
Feasibility of Miscanthus as alternative bedding for dairy cowsOriginal Paper
S. Van Weyenberg, T. Ulens, K. De Reu, I. Zwertvaegher, P. Demeyer, L. Pluym
Vet Med - Czech, 2015, 60(3):121-132 | DOI: 10.17221/8059-VETMED
Despite dairy farmers' awareness of the importance of correct bedding for the health and comfort of their cows, they are often frugal with respect to these bedding materials in order to reduce costs. In addition, farmers are currently dependent on the availability and price fluctuations of traditional bedding materials. For these reasons, the scientific literature as well as the trade press point to an intensifying search for affordable alternative bedding materials. The aim of this study was to investigate whether Miscanthus, a woody grass that requires low input but generates high yield, could replace straw in deep little dairy cow cubicles....
The genotoxicity of caecal water from gilts following experimentally induced Fusarium mycotoxicosisOriginal Paper
A. Nowak, K. Slizewska, M. Gajecka, M. Piotrowska, Z. Zakowska, L. Zielonka, M. Gajecki
Vet Med - Czech, 2015, 60(3):133-140 | DOI: 10.17221/8058-VETMED
The examination of caecal contents allows one to investigate the exposure of colon mucosa to dietary toxins and the chemical contaminants present in animal feed. The objective of the present study was to examine the influence of Fusarium mycotoxins (zearalenone, ZEN, and deoxynivalenol, DON), administered separately and as a mixture, on the genotoxicity of caecal water (CW) from gilts fed these mycotoxins. CW genotoxicity was evaluated with the comet assay using the LLC-PK1 porcine epithelial kidney cell line. It was shown that after the first week of the experiment, the presence of DON in animal feed led to increased CW genotoxicity in the...
Primary screening of potentially bio-active substances in the lyophilisate of Pectinatella magnifica biomassOriginal Paper
Z. Balounova, V. Brezina, K. Susterova, J. Rajchard
Vet Med - Czech, 2015, 60(3):141-146 | DOI: 10.17221/8060-VETMED
The purpose of this research was to examine potential bio-active substances in the lyophilisate of Pectinatella magnifica biomass and its focus was on primary screening of the biomass. Extracts of lyophilisate were applied to murine cells and their effects on cell population growth, cell morphology and cell distribution were monitored. The inhibition of cytoskeleton repair reached its maximum after a 24-h exposure of the cells to the extract prepared at a temperature of 37 °C, whereas the 1-h application of the extract leached at a temperature of 37 °C resulted in quick repair of cytoskeletal function. When compared with the control, in which...
Hormonal response of Arctic fox females to short- and long-term stressOriginal Paper
E. Gorajewska, A. Filistowicz, S. Nowicki, P. Przysiecki, A. Filistowicz, K. Czyz
Vet Med - Czech, 2015, 60(3):147-154 | DOI: 10.17221/8061-VETMED
The aim of this study was to determine the extent of the hormonal response of Arctic fox females exposed to two types of stress: short-term and long-term, combined with adaptation to new environmental conditions. Short-term stress (20 min) was investigated in 34 females on farm A in the Wielkopolska province. The testing procedure involved capturing of the animals, their immobilisation, phenotype evaluation, and placing in target cages. Blood for laboratory examinations was collected from the vena cephalica antebrachii three times: on the day of the test, directly after the procedure and after three days. Long-term stress (8 h), combined with...
The effect of deslorelin acetate on the oestrous cycle of female guinea pigsOriginal Paper
S. Kohutova, V. Jekl, Z. Knotek, K. Hauptman
Vet Med - Czech, 2015, 60(3):155-160 | DOI: 10.17221/8062-VETMED
Deslorelin acetate, a GnRH agonist, is commonly used to prevent folliculogenesis in several species. However, little is known of the effect of deslorelin acetate implants on the oestrous cycle of female guinea pigs. Fifteen intact healthy female guinea pigs were investigated in this study. Signs of sexual behaviour, the presence of a vaginal membrane along with plasma concentrations of oestradiol (E2)and progesterone (P4), were monitored during two consecutive oestrous cycles. At the beginning of the third oestrous cycle each guinea pig was administered an implant of the GnRH analogue, deslorelin acetate, (4.7 mg). When compared...
Analysis of morphological variation of the internal ophthalmic artery in the chinchilla (Chinchilla laniger, Molina)Original Paper
J. Kuchinka
Vet Med - Czech, 2015, 60(3):161-169 | DOI: 10.17221/8063-VETMED
The aim of this investigation was the analysis of the variability within the internal and external ophthalmic artery in the chinchilla (Chinchilla laniger, Molina). The head vasculature of 65 individuals was analysed, with particular emphasis on the internal ophthalmic artery originating from the central and rostral part of the cerebral arterial circle. Head blood vessels were filled with acrylic latex for vascular corrosion casting. The results showed ten variants of blood supply for the orbit, with a predominance of the first variant (66.1%) = bilateral presence of the external ophthalmic artery originating from the maxillary artery. Other...
SRY-negative XX sex reversal in an English Cocker Spaniel: a case reportCase Report
D. Nak, T. Gulten, M. Karkucak, R. Yilmaz, Y. Nak, G. Simsek, A.H. Shahzad
Vet Med - Czech, 2015, 60(3):170-173 | DOI: 10.17221/8064-VETMED
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome which supports the hypothesis that some other genes are also responsible for testicular development. The present case report describes a one-year-old dog brought to the hospital with a complaint of no oestrus cycle. Apparently normal masculine bitch had an enlarged clitoris with an os on radiograph. A gonado-hysterectomy was performed. Instead of ovarian structures both testicular masses were present and a normal complete bicornuate uterus with a normal...