Veterinární medicína, 2017 (vol. 62), issue 5

Heart rate variability in Mangalarga Marchador horses after physical exerciseOriginal Paper

C.S. Coelho, D. Frederico, L.A.T. Oliveira Jr., M.B. Binda, L.M.C. Conti, T. Champion

Vet Med - Czech, 2017, 62(5):239-244 | DOI: 10.17221/139/2016-VETMED  

The aim of this study was to evaluate the influence of physical exercise (marcha gait) on heart rate variability indices in Mangalarga Marchador horses. Twenty-five animals, fourteen females and eleven males, aged 6.4 ± 2.7 years and with a mean weight of 428.3 ± 24.7 kg, were used. Electrocardiograms were recorded at two different time points: rest and immediately after exercise (40 min of aerobic exercise, marcha gait). Time domain analyses of heart rate variability were made using vasovagal tonus index (VVTI) and standard deviation of normal intervals (SDNN). Variables were analysed for normality using the Shapiro-Wilk test and comparisons...

Positive effects of trehalose and cysteine on ram sperm parametersOriginal Paper

S. Gungor, C. Ozturk, A.D. Omur

Vet Med - Czech, 2017, 62(5):245-252 | DOI: 10.17221/131/2016-VETMED  

The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial...

Expression profile of the SOX9 gene in the testes of sexually immature and mature male goats (Capra hircus), and its potential influence on postnatal testis developmentOriginal Paper

I. Szatkowska, M. Jedrzejczak-Silicka, A. Dybus, B. Wiszniewska, J. Udala, D. Zaborski, J. Wojcik, T. Stankiewicz, W.S. Proskura

Vet Med - Czech, 2017, 62(5):253-260 | DOI: 10.17221/147/2016-VETMED  

The aim of this study was to compare the expression levels of the SOX9 (SRY-box 9) gene in the testes of 18 White improved male goats (Capra hircus) divided into three age groups (one, 10 and 15 months of age with seven, eight and three individuals per group, respectively). Abnormalities in testis development were observed in three individuals from the group of 10-month-olds. Additionally, differences in SOX9 expression that may affect the process of testis maturation and testicular spermatogenic activity were investigated among individuals. The expression of the SOX9 gene in testicular tissues was analysed using...

The effect of orally administered Budesonide on the hypothalamic-pituitary-adrenal axis in dogs with inflammatory bowel diseaseOriginal Paper

A. Rychlik, M. Nowicki, A. Kolodziejska-Sawerska, M. Szweda

Vet Med - Czech, 2017, 62(5):261-268 | DOI: 10.17221/130/2015-VETMED  

The aim of this study was to evaluate the effect of Budesonide on the hypothalamic-pituitary-adrenal (HPA) axis in dogs with inflammatory bowel disease. The effect of orally administered Budesonide (Entocort) on the HPA axis was analysed in 21 dogs with inflammatory bowel disease. Biochemical analyses were carried out to evaluate the activity levels of alanine aminotransferase, asparagine aminotransferase, alkaline phosphatase, cortisol and endogenous adrenocorticotropic hormone. Urine samples were collected from each patient before the study and after 30 days of the experiment to determine the composition and the physical and chemical properties of...

A survey of feline trichomonosis suggests a low incidence of Tritrichomonas blagburni among cats in the Czech RepublicOriginal Paper

V. Ceplecha, V. Svobodova, C. Lendon, R. Husnik, K. Horackova, M. Svoboda

Vet Med - Czech, 2017, 62(5):269-273 | DOI: 10.17221/106/2016-VETMED  

Tritrichomonas blagburni (previously called T. foetus) has been implicated as an aetiological agent of long-term large-bowel diarrhoea in cats in many countries worldwide. The aim of this study was to determine the presence of, and risk factors for T. blagburni among a cohort of cats living in different conditions in the Czech Republic. Samples were collected from 170 cats living in different environments. The InPouch™ TF-Feline medium method was used for diagnosis of feline trichomonosis. A single case (0.6%) with motile trichomonads identified as Pentatrichomonas hominis was found in a cat...

Evaluation of the contraceptive effects of carprofen, flunixin meglumine and meloxicam in ratsOriginal Paper

Z. Paksoy, A. Kirbas

Vet Med - Czech, 2017, 62(5):274-278 | DOI: 10.17221/67/2016-VETMED  

The objective of this study was to determine the suitability of carprofen, flunixin meglumine and meloxicam for use in emergency contraception. Forty-eight pregnant Sprague-Dawley rats were used as material. Five groups were subjected to treatments while one group served as a control. The numbers of animals in each group were equal (n = 8). Treatment groups were administered carprofen (10 mg/kg, single or double dose, s.c.), flunixin meglumine (5 mg/kg, single or double dose, i.m.) and meloxicam (2 mg/kg, a single dose, s.c.) on the third day after mating. The control group received saline. The rats were sacrificed...

Double symmetrical aortic arch in a Czechoslovakian wolfdog - surgical treatment and health assessment four years after treatment: a case reportCase Report

P. Skrzypczak, A. Piatek, J. Biezynski, Z. Kielbowicz

Vet Med - Czech, 2017, 62(5):279-284 | DOI: 10.17221/274/2015-VETMED  

The paper describes a double symmetrical aortic arch in a dog trapping the oesophagus. Thoracotomy was performed in the fourth inter-costal space under general, isoflurane-maintained, anaesthesia. Cutting of the arterial ligament failed to free the oesophagus. Dissection of further vascular structures revealed the left and right aortic arches to be of similar diameter. A vascular clamp was put on the left arch for 10 min, while the patient's cardiovascular parameters were monitored. The lack of significant cardio-respiratory symptoms indicated the necessity of ligating and cutting the left aortic arch. Removal of additional tissue from the region of...

Comparison of continuous and intermittent glucose monitoring systems in a dog with diabetic ketoacidosis: a case reportCase Report

W. Kim, H. Kim, S. Kang, J. Bae, J. Choi, J. Park, D. Yu

Vet Med - Czech, 2017, 62(5):285-291 | DOI: 10.17221/25/2017-VETMED  

A Miniature schnauzer (12 years old, neutered male) was referred for lethargy, anorexia, and oral bleeding. On initial evaluation, severe hyperglycaemia (blood glucose concentration of 34.9 mmol/l), ketonuria, systemic inflammation (fever, panting, left-shift neutrophilia, and a high C-reactive protein level of 980.97 nmol/l, abnormal pancreatic lipase immunoreactivity, and periodontitis were found. With consideration of possible insulin resistance, blood glucose (BG) levels were monitored using a portable glucose meter (q 1-3 h) and a continuous glucose monitoring system (CGMS) for 72 h (three consecutive trials); intensive insulin therapy...

Granulomatous colitis in two French bulldogs unresponsive to fluoroquinolone antimicrobials: a case reportCase Report

R. Lucena, M. Novales, P.J. Ginel

Vet Med - Czech, 2017, 62(5):292-294 | DOI: 10.17221/49/2016-VETMED  

Two cases of granulomatous colitis in two French bulldogs were found to be unresponsive to fluoroquinolones. The granulomatous colitis diagnosis was made on the basis of PAS-positive histiocytes in the lamina propria of the colonic mucosa in biopsy samples taken at colonoscopy. Remission of granulomatous colitis has been reported using fluoroquinolones leading to the idea that invasive Escherichia coli strains in the colonic mucosa are involved. Oral enrofloxacin (Baytril 150 mg, Bayer, Spain) at 10 mg/kg per day for eight weeks was prescribed to both dogs in this study. A first course of therapy resolved the problem in dog No. 1, which, however,...

Recurrent feline gastrointestinal eosinophilic sclerosing fibroplasia and presumptive eosinophilic cystitis in a domestic short-haired cat: a case reportCase Report

M. Brloznik, S. Faraguna, M. Gombac, T. Svara

Vet Med - Czech, 2017, 62(5):295-300 | DOI: 10.17221/134/2016-VETMED  

This report describes recurrent feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) and eosinophilic cystitis in an 8-year-old domestic short-haired cat. The cat presented with incontinence, pollakiuria, vomiting and weight loss. An abdominal mass was palpated and ultrasonography revealed severe segmental thickening of the jejunal wall with loss of layering. Complete resection of the jejunal mass was performed. The resected mass was composed of branching trabeculae of dense collagen, intermixed with bundles of large fibroblasts and inflammatory cells, which is consistent with FGESF. One year later, a new mass reoccurred in another intestinal...