Vet Med - Czech, 2024, 69(8):297-306 | DOI: 10.17221/21/2024-VETMED

Therapeutic potential of cannabis for surgical wound healing in ratsOriginal Paper

GD Akarsu ORCID...1,2, R Hobek Akarsu ORCID...3
1 Laboratory for Advanced Genomics, Division of Molecular Medicine, Institute of Ruder Boskovic, Zagreb, Croatia
2 Pharmacy Services, Vocational School of Health Services, Yozgat Bozok University, Yozgat, Turkiye
3 Department of Nursing, Faculty of Health Services, Yozgat Bozok University, Yozgat, Turkiye

This study was conducted to evaluate the wound-healing activities of a Cannabis sativa L. plant extract and cannabidiol on incision wounds. An incision was created and sutured in rats under anaesthesia. Routine wound care procedures were applied for 10 days, followed by histological wound examinations. The cellular bioactivities of the hemp extract and CBD were assessed for MCP-1, EGF, BFGF, IL-8, and COL-1 using ELISA on the rat skin wound healing activity. A one-way ANOVA was used for the data analysis. The EGF values in the plasma were similar in the povidone-iodine, hemp seed oil, and hemp essential oil groups (P > 0.05). However, the EGF levels were lower in the CBD group compared to the other groups (P < 0.001, P < 0.005). The MCP-1 values in the hemp seed oil, hemp essential oil, and CBD were similar (P > 0.05), whereas povidone iodine exhibited lower MCP-1 levels compared to the other groups (P < 0.001, P < 0.005). It was determined that the plasma BFGF, IL-8, and COL 1 values of the groups were similar (P > 0.05). To our knowledge, this study is the first to evaluate the effects of CBD, seed oil, and hemp leaf extract on incision wound healing. It demonstrates that hemp extract holds greater potential benefits for wound healing compared to CBD.

Keywords: cannabinoids; cannabis sativa; essential oil; hemp; treatment; wounds and injury

Received: March 5, 2024; Revised: May 22, 2024; Accepted: May 28, 2024; Prepublished online: August 16, 2024; Published: August 29, 2024  Show citation

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Akarsu G, Hobek Akarsu R. Therapeutic potential of cannabis for surgical wound healing in rats. Vet Med - Czech. 2024;69(8):297-306. doi: 10.17221/21/2024-VETMED.
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