Vet Med - Czech, 2001, 46(5):149-152 | DOI: 10.17221/7871-VETMED

Qualitative and quantitative cytometric analysis of peripheral blood leukocytes in carps (Cyprinus carpio)Original Paper

M. Stosik, W. Deptuła, K. Wiktorowicz, M. Trávniček, K. Baldy-Chudzik
1 Higher Pedagogical School, Zielona Góra, Poland
2 University of Szczecin, Szczecin, Poland
3 Medical School, Poznañ, Poland
4 University of Veterinary Medicine, Košice, Slovak Republic

The studies were performed involving qualitative and quantitative cytometric analysis of peripheral blood leukocytes in healthy carps at various stages of their ontogeny, i.e., in 3- to 29-month old carps. Three populations of leukocyte line cells were distinguished, which differed in forward scatter (FSC) and side scatter (90o, SSC) of laser light. The most abundant leukocyte pool was present in the eldest (23- to 29-month old) fish. Lower numbers of the cells were observed in the youngest (3- to 9-month old) carps while the lowest levels of the cells were detected in carps of the moderate age (11- to 21-month old). The leukocyte populations, distinguished on grounds of FSC/SSC characteristics, were suggested to correspond to lymphocytes/thrombocytes (low FSC, low SSC), granulocytes (high FSC, high SSC) and monocytes (high FSC, low SSC).

Keywords: carp; leukocytes; cytometry

Published: May 31, 2001  Show citation

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Stosik M, Deptuła W, Wiktorowicz K, Trávniček M, Baldy-Chudzik K. Qualitative and quantitative cytometric analysis of peripheral blood leukocytes in carps (Cyprinus carpio). Vet Med - Czech. 2001;46(5):149-152. doi: 10.17221/7871-VETMED.
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    References

    1. Bly J., Miller N.W., Clem L.W. (1990): A monoclonal antibody specific for neutrophils in normal and stressed channel catfish. Dev. Comp. Immunol., 14, 211–221. Go to original source... Go to PubMed...
    2. Chilmoñczyk S., Monge D. (1999): Flow cytometry as a tool for assessment of the fish cellular immune response to pathogens. Fish Shelfish Immunol.. 9, 319. Go to original source...
    3. Chilmoñczyk S., Voccia I., Monge D. (1995): Pathogenesis of viral haemorrhagic septicemia virus: cellular aspects. Vet. Res., 26, 501–511.
    4. Chilmoñczyk S., Voccia I., Tarazona J.V., Monge D. (1997): Flow cytometric analysis of fish leucocyte populations exposed to pollutants and pathogens: modulatory effects induced by experimental procedures. In: Zelikoff J.T. (ed.): Ecotoxicology: Responses, Biomarkers and Risk Assessment. SOS Publications, Fair Haven. 171–184.
    5. DeLuca D., Wilson M., Warr G. (1983): Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM. Eur. J. Immunol., 13, 546–551. vitro quantitative kinetic study of infectious pancreatic necrosis viral antigen by flow cytometry. Fish Pathol., 30, 1–5. Go to original source... Go to PubMed...
    6. Secombes C.J., Fletcher T.C. (1992): The role of phagocytes in the protective mechanisms of fish. Ann. Rev. Fish Dis., 2, 53–71. Go to original source...
    7. Siegl E., Nebe B., Blunk H., Rychly J. (1998): Detection of mitogen induced stimulation of leukocytes from the rainbow trout (Oncorhynchus mykiss) by flow cytometric analysis of intracellular calcium. Comp. Biochem. Physiol., 119, 915–923. Go to original source...
    8. Slierendrecht W.J., Lorenzen N., Glamann J., Koch C., Rombout J.H.W.M. (1995): Immunocytochemical analysis of a monoclonal antibody specific rainbow trout (Oncorhynchus mykiss) granulocytes and thrombocytes. Vet. Immunol. Immunopathol., 46, 349–360. Go to original source... Go to PubMed...
    9. Stosik M. (1995): Selected immunological and haematological parameters in culturedhealthy and sick carps (Cyprinus carpio L.) in the course of their ontogeny (in Polish). [Thesis of habilitated doctor.] Scientific Editorial Office, University of Szczecin, Szczecin.
    10. Voccia I., Krzystyniak K., Flipo D., Fournier M.(1994): In vitro mercury-related cytotoxicity and functional impairment of the immune cells of rainbow trout (Oncorhynchus mykiss). Aquatic Toxicol., 29, 37–48. Go to original source...
    11. Vowells S.J., Sekhsaria S., Malech H.L., Shalit M., Fleisher T.A. (1995). Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes.
    12. J. Immunol. Methods, 13, 89–97.

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