Veterinární medicína, 2001 (vol. 46), issue 5

Monoclonal antibodies to bovine coronavirus and their use in enzymoimmunoanalysis and immunochromatographyOriginal Paper

S. Reschová, D. Pokorová, Z. Nevoránková, J. Franz

Vet Med - Czech, 2001, 46(5):125-131 | DOI: 10.17221/7869-VETMED  

Two monoclonal antibodies (MAb) to the outer structural protein E2 (spike peplomeric protein) and two MAb to the inner capsid protein N of bovine coronavirus (BCV) were prepared and identified by Western blotting to be used for increasing the specificity and sensitivity of BCV detection. The MAb were checked by the haemagglutination inhibition test and immunoperoxidase tests and no cross reactivity with rotavirus was demonstrated by the immunoperoxidase test and ELISA. A mixture of all the four MAb at predetermined optimum concentrations was first used in sandwich ELISA and then, in combination with an anti‑coronavirus polyclonal antibody, for...

Variations in contamination by mercury, cadmium and lead on swine farms in the district of Hodonin in 1994 to 1999Original Paper

R. Ulrich, J. Raszyk, A. Nápravník

Vet Med - Czech, 2001, 46(5):132-139 | DOI: 10.17221/7872-VETMED  

Environmental samples (n = 254) were collected at regular intervals from 1994 to 1999 on three swine farms in the area of the district town Hodonín, Czech Republic. The samples of feed mixtures (n = 85), barn dust sediments (n = 44), and muscular (n = 42), hepatic (n = 41), and renal (n = 42) tissues of feeder pigs were analysed for the concentrations of mercury, cadmium and lead. The concentrations of mercury showed decreasing tendencies in animal tissue samples (1994-1996). Compared with the mean for the Czech Republic, the concentration of mercury was markedly higher in liver and kidney samples...

Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stressOriginal Paper

K. Sahin, N. Sahin, M. Onderci, S. Yaralioglu, O. Kucuk

Vet Med - Czech, 2001, 46(5):140-144 | DOI: 10.17221/7870-VETMED  

An experiment utilizing Cobb-500 male broilers was conducted to evaluate the effects of vitamin E supplementation at various concentrations on malonyldialdehyde (MDA) as an indicator of lipid peroxidation, serum and liver concentrations of antioxidant vitamins and some minerals of broilers reared under heat stress (32°C). One day-old 150 male broilers were randomly assigned to 5 treatment groups, 3 replicates of 10 birds each. The birds received either a basal diet or basal diet supplemented with vitamin E (dl-a-tocopherol acetate) at 62.5, 125, 250, or 500 mg/kg of diet. Increased supplemental vitamin E linearly increased serum vitamin E and A, but...

Changes in serum concentration of 17 beta-estradiol in female rats during estrous cycle after treatment with atrazine and zearalenoneOriginal Paper

M. Mitak, T. Gojmerac, B. Mandić, Ž. Cvetnić

Vet Med - Czech, 2001, 46(5):145-148 | DOI: 10.17221/7868-VETMED  

A daily dose of 14 mg atrazine and 2.5 mg zearalenone, given p.o. during 5 days of estrous cycle to female rats, changed their estrous cycle in comparison with control animals. On day -1 of expected estrus, a significantly lower (p < 0.05) concentration of 17b-estradiol compared with the control group was recorded in all experimental groups of animals. In the group of animals administered zearalenone, the concentration of 17b-estradiol on the day of expected estrus was significantly higher (p < 0.05). In the group administered a combination of atrazine and zearalenone, the concentration of 17b-estradiol on the day after...

Qualitative and quantitative cytometric analysis of peripheral blood leukocytes in carps (Cyprinus carpio)Original Paper

M. Stosik, W. Deptuła, K. Wiktorowicz, M. Trávniček, K. Baldy-Chudzik

Vet Med - Czech, 2001, 46(5):149-152 | DOI: 10.17221/7871-VETMED  

The studies were performed involving qualitative and quantitative cytometric analysis of peripheral blood leukocytes in healthy carps at various stages of their ontogeny, i.e., in 3- to 29-month old carps. Three populations of leukocyte line cells were distinguished, which differed in forward scatter (FSC) and side scatter (90o, SSC) of laser light. The most abundant leukocyte pool was present in the eldest (23- to 29-month old) fish. Lower numbers of the cells were observed in the youngest (3- to 9-month old) carps while the lowest levels of the cells were detected in carps of the moderate age (11- to 21-month old). The leukocyte populations, distinguished...