Vet Med - Czech, 2003, 48(8):221-228 | DOI: 10.17221/5773-VETMED

Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity

Z. Veznik, D. Svecova, A. Zajicova, P. Prinosilova
Veterinary Research Institute, Brno, Czech Republic

38 ejaculates of dogs of various breeds from2to 6 years old were examined in a short-term 120 min survival test. The examination focused on the functional parameters of sperm (motility, percentage of live sperma­tozoa, acrosomal integrity) and their morphology. The levels of relationship between individual criteria of sperm analysis were demonstrated. Statistically highly significant correlations between motility, ratio of live spermatozoa, percentage of intact acrosomes and percentage of spermatozoa with altered superficial structures were proven. Our findings proved the sensitivity of superficial structures, primarily plasmatic membranes, and the higher resistance of structures with an organelle basis. Strict morphological sperm analysis provides important information about the rise in qualitative alterations of ejaculates caused by changes in superficial structures during the course of a short-term survival test.

Keywords: dog; ejaculate; sperm analysis; membrane and acrosome integrity

Published: August 31, 2003  Show citation

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Veznik Z, Svecova D, Zajicova A, Prinosilova P. Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity. Vet Med - Czech. 2003;48(8):221-228. doi: 10.17221/5773-VETMED.
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    References

    1. Ashworth P.J.C., Harrison R.A.P., Miller N.G.A., Plummer J.M., Eatson P.F. (1995): Flow cytometric detection of bicarbonate-induced changes in lectin binding in boar and ram sperm populations. Mol. Reprod. Dev., 40, 164-176. Go to original source... Go to PubMed...
    2. Baker F., Gragle R.G., Salisbury G.W., Van Demark N.L. (1957): Spermatozoa velocities in vitro. A simple method of measurement. Fe il. Steril., 8, 149-155. Go to original source... Go to PubMed...
    3. Chong A.P., Walters C.A., Weinrieb S. (1983): The neglected laboratory test. J. Androl., 4, 280-282. Go to original source... Go to PubMed...
    4. Christiansen I.J. (1984): Reproduction in the dos and cat. Bailliere Tindall. London, 99-100.
    5. Dahlbom M., Andersson M., Juga J., Alanko M. (1997): Fertility parameters in male Irish wol ounds: a twoyear follow-up study. J. Small Anim. Pract., 12, 547- 550. Go to original source...
    6. Deibel F.C.Jr., Smith J.E., Crabo B.G., Graham E.F. (1976): Evaluation of six assays of sperm quality by means of their accuracy, precision and sensitivity in separating know induced levels of damage. In: Proc. 8th Int. Congr. Anim. Reprod. an AI, 4, 888-891.
    7. Dunphy B.C., Kay R., Barrat C.L.R., Cooke I.D. (1989): Quality control during the conventional analysis of sperm, an essential exercise. J. Androl., 10, 378-385. Go to original source... Go to PubMed...
    8. Geussova G., Peknicova J., Capkova J., Kalab P., Moos J., Philimonenko V.V., Hozak P. (1997): Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia, 5, 261-268. Go to original source... Go to PubMed...
    9. Guerin P., Ferrer M., Fontbonne A., Benigni L., Jacquet M., Menezo Y. (1999): In vitro capacitation of dog spermatozoa as assessed by chlortetracycline staining. Theriogenology, 4, 617-628. Go to original source... Go to PubMed...
    10. Iguer-Ouada M., Verstagen J.P. (2001a): Validation of the sperm quality analyser (SQA) for dog sperm analysis. Theriogenology, 55, 1143-1158. Go to original source... Go to PubMed...
    11. Iguer-Ouada M., Verstagen J.P. (2001b): Evaluation of the Hamilton Thorn computer - based automated system for dog semen analysis. Theriogenology, 55, 733-749. Go to original source... Go to PubMed...
    12. Jeyendran R.S., Van Ded Ven H.H., Perez-Pelaez M., Crabo B.G., Zaneveld L.J.D. (1984): Development of an assay to asses the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70, 219-228. Go to original source... Go to PubMed...
    13. Jeyendran R.S., Van der Ven H.H., Zaneveld L.J.D. (1992): The hypoosmotic swelling test; an update. Arch. Androl., 29, 105-116. Go to original source... Go to PubMed...
    14. Kawakami E, Hori T, Tsutsui T. (2000): Azoospermia of dogs with apoptotic germ cells and Leydig cells. J. Vet. Med. Sci., 5, 529-531. Go to original source... Go to PubMed...
    15. Koehler J.K., Platz C.C. Jr, Waddell W., Jones M.H., Behrns S. (1998): Semen parameters and electron microscope observations of spermatozoa of the red wolf, Canis rufus. J. Reprod. Fertil., 1, 95-101. Go to original source... Go to PubMed...
    16. Matouskova O., Cigler M., Chalupa J., Hruska K. (1992): STAT Plus - Manual (in Czech). 1st ed. Veterinary Research Institute, Brno. 168 pp.
    17. Menkveld R., Kruger T.F. (1995): Advantages of strict (Tygerberg) criteria for evaluation of sperm morphology. Int. J. Androl., 2, 36-42.
    18. Nothling J.O., Gerstenberg C., Volkmann D.H. (1997): Semen quality a er thawing: correlation with fertility and fresh semen quality in dogs. J. Reprod. Fertil., 51, 109-116.
    19. Oe le E.E. (1993): Sperm morphology and fertility in the dog. J. Reprod. Fertil. 47, Suppl., 257-260. Go to PubMed...
    20. Pena A., Linde-Forsberg C. (2000a): Effects of spermatozoal concentration and post-thaw dilution rate on survival a er thawing of dog spermatozoa. Theriogenology, 54, 703-718. Go to original source... Go to PubMed...
    21. Pena A., Linde-Forsberg C. (2000b): Effects of Equex, one- or two-step dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa. Theriogenology, 54, 859-875. Go to original source... Go to PubMed...
    22. Pena A., Johannisson A., Linde-Forsberg C. (1999): Postthaw evaluation of dog spermatozoa using new triple fluorescent staining and flow cytometry. Theriogenology, 52, 965-980. Go to original source... Go to PubMed...
    23. Rigau T., Farré M., Ballester J., Mogas T., Pena A., Rodrigez-Gil J.E. (2001): Effects of glucose and fructose on motility pa erns of dog spermatozoa from fresh ejaculates. Theriogenology, 56, 801-815. Go to original source... Go to PubMed...
    24. Root Kustritz M.V., Olson P.N., Johnston S.D., Root T.K. (1998): The effects of stains and investigators on assessment of morphology of canine spermatozoa. J. Am. Anim. Hosp. Assoc., 4, 348-352. Go to original source...
    25. Schafer S., Holzman A., Arbeiter K. (1996): Modification of the quality indicators of the ejaculate of beagle dogs due to frequent collections over time. Tierarztl. Prax., 24, 385-390. Go to PubMed...
    26. Sirivaidyapong S., Bevers M.M., Colenbrander B. (1999): Acrosome reaction in dog sperm is induced by a membrane-localized progesterone receptor. J. Androl., 4, 537-544. Go to original source...
    27. Sirivaidyapong S., Cheng F.P., Marks A., Voorhout W.F., Bevers M.M., Colenbrander B. (2000): Effect of sperm diluents on the acrosome reaction in canine sperm. Theriogenology, 3, 789-802. Go to original source... Go to PubMed...
    28. Szasz F., Sirivaidyapong S., Cheng F.P., Marks A., Voorhout W.F, Marks A., Colenbrander B., Solti A.L., Gadella B.M. (2000): Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen. Mol. Reprod. Dev., 55, 289-298. Go to original source... Go to PubMed...
    29. Veznik Z. (1970): Využití morfologických ukazatelů ke kvalitativnímu hodnocení ejakulátů. Vet. Med. Praha, 15, 571-578.
    30. Veznik Z., Svecova D. (1992): Soubor metod k hodnocení pohlavních funkcí býků, ÚVO Pardubice, 184 s.
    31. Veznik Z., Svecova D., Zajicova A. et al. (2000): Hodnocení semene pro asistovanou reprodukci a výběr plemeníků.

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