Vet Med - Czech, 2016, 61(10):546-552 | DOI: 10.17221/51/2016-VETMED

Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogsOriginal Paper

R. Ciaputa, P. Bandoch, K. Lewandowska, J.A. Madej, M. Kandefer-Gola, I. Janus, M. Nowak
Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies against carcino-embryonic antigen (CEA) cytokeratin (CK) 7 and 20, CD4, CD8, minichromosome maintenance protein 3 (MCM3), vimentin, and alpha 1 foetoprotein (AFP). In addition, Mallory's connective tissue stain, van Gieson's stain and Gomori methenamine silver stain were used. The study was performed on liver samples collected post mortem from five mixed-breed dogs aged 9-12 years. The tumours were classified according to the World Health Organization Classification of Tumours. Strong expression of MCM3 and AFP was found in the hepatic cancer cells and in the metastases to the lungs, spleen and kidneys. The primary tumours and metastatic foci did not react positively with the anti-CD4, anti-CD8, CEA, CK7 and CK20 antibodies. The connective tissue in the primary tumour and the metastases showed a positive reaction to vimentin. Canine hepatocellular carcinomas that metastasise are highly-malignant well-differentiated tumours that produce AFP and trace amounts of both carcinoembryonic antigen and cytokeratin. Therefore, the metastasis resembles the primary tumour and has a common phenotype and genotype with the primary tumour.

Keywords: cell markers; expression; CEA; CK7; CK20; CD4; CD8; vimentin; AFP; immunohistochemistry; pleomorphic cells; liver

Published: October 31, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ciaputa R, Bandoch P, Lewandowska K, Madej JA, Kandefer-Gola M, Janus I, Nowak M. Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogs. Vet Med - Czech. 2016;61(10):546-552. doi: 10.17221/51/2016-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Center SA, Slater MR, Manwarren T, Prymak K (1992): Diagnostic efficacy of serum alkaline phospathase and γ-glutamyltransferase in dogs with histologically confirmed hepatobiliary disease: 270 cases (1980-1990). Journal of the American Veterinary Medical Association 201, 1258-1265. Go to original source...
    2. Clayton EF, Furth EE, Ziober A, Xu T, Yao Y, Hwang PG, Bing Z (2012): A case of primary clear cell hepatocellular carcinoma in a non-cirrhotic liver: an immunohistochemical and ultrastructural study. Rare Tumors 4, 29. Go to original source... Go to PubMed...
    3. de las Mulas JM, Gomez-Villamandos JC, Perez J, Mozos E, Estrada M, Mendez A (1995): Immunohistochemical evaluation of canine primary liver carcinomas: distribution of alpha-fetoprotein, carcinoembryonic antigen, keratins and vimentin. Research in Veterinary Science 59, 124-127. Go to original source... Go to PubMed...
    4. Dellagi K, Vainchenker W, Vinci G, Paulin D, Brouet J, Cembo J (1983): Alteration of vimentin intermediate filament expression during differentiation of human hemopoietic cells. The EMBO Journal 2, 1509-1514. Go to original source... Go to PubMed...
    5. EASL-EORTC - European Association for the Study of the Liver, European Organisation for Research and Treatment of Cancer (2012): EASL-EORTC Clinical practice guidelines: Management of hepatocellular carcinoma. European Association for the Study of the Liver, European Organisation for Research and Treatment of Cancer. Journal of Hepatology 56, 908-943. Go to original source... Go to PubMed...
    6. Endl E, Kausch I, Baack M, Knippers R, Gerdes J, Scholzen T (2001): The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells. Journal of Pathology 195, 457-462. Go to original source... Go to PubMed...
    7. Hamilton SR, Aaltonen LA (2000): 8 Tumours of the liver and intrahepatic bile ducts. In: Kleihues P, Sobin LH (eds): World Health Organization Classification of Tumours: Pathology and Genetics of Tumours of the Digestive System. IARC Press, Lyon. 157-199.
    8. Handra-Luca A, Hong SM, Walter K, Wolfgang C, Hruban R, Goggins M (2011): Tumour epithelial vimentin expression and outcome of pancreatic ductal adenocarcinomas. British Journal of Cancer 104, 1296-1302. Go to original source... Go to PubMed...
    9. Jia SQ, Ren JJ, Dong PD, Meng XK (2013): Probing the hepatic progenitor cell in human hepatocellular carcinoma. Gastroenterology Research and Practice, DOI: 10.1155/ 2013/145253. Go to original source... Go to PubMed...
    10. Kapatkin AS, Mullen HS, Matthiesen DT, Patnaik AK (1992): Leiomyosarcoma in dogs: 44 cases (1983-1988). Journal of the American Veterinary Medical Association 201, 1077-1079. Go to original source...
    11. Kitao S, Yamada T, Ishikawa T, Madarame H, Furuichi M, Neo S, Tsuchiya R, Kobayashi K (2006): Alpha-fetoprotein in serum and tumor tissues in dogs with hepatocellular carcinoma. Journal of Veterinary Diagnostic Investigation 18, 291-295. Go to original source... Go to PubMed...
    12. Krus S, Skrzypek-Fakhoury E (1996): 12 Morphological background of insufficiency of liver and biliary system (in Polish). In: Krus S, Skrzypek-Fakhoury E (eds): Clinical Pathomorphology (in Polish). 3rd edn. Wydawnictwo Lekarskie PZWL, Warszawa. 465-531.
    13. Lahat G, Zhu QS, Huang KL, Wang S, Bolshakov S, Liu J, Torres K, Langley RR, Lazar AJ, Hung MC, Lev D (2010): Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies. PLoS One, DOI: 10.1371/journal.pone.0010105. Go to original source...
    14. Lee JW, Kim MW, Choi NK, Cho IJ, Hong R (2014): Double primary hepatic cancer (sarcomatoid carcinoma and hepatocellular carcinoma): A case report. Molecular and Clinical Oncology 2, 949-295. Go to original source... Go to PubMed...
    15. Leibman NF, Larson VS, Ogilvie GK (2003): 11 Oncologic diseases of the digestive system. In: Tams TR (ed.): Handbook of Small Animal Gastroenterology. Saunders, St Louis. 370-415. Go to original source...
    16. Leong ASY, Cooper K, Leong FJWM (1999): Manual of diagnostic antibodies for immunohistology. Greenwich Medical Media Ltd., London. 385 pp.
    17. Liptak JM, Dernell WS, Monnet E, Powers BE, Bachand AM, Kenney JG, Withrow SJ (2004): Massive hepatocellular carcinoma in dogs: 48 cases (1992-2002). Journal of the American Veterinary Medical Association 225, 1225-1230. Go to original source... Go to PubMed...
    18. Maliszewski D, Jastrzebski T, Drucis K, Kopacz A (2008): Prognostic factors in colon cancer - what can we add to the standards? Wspolczesna Onkologia 5, 212-216.
    19. Masserdotti C, Drigo M (2012): Retrospective study of cy
    20. Nyland TG, Koblik PD, Tellyer S (1999): Ultrasonographic evaluation of biliary cystadenomas in cats. Veterinary Radiology and Ultrasound 40, 300-306. Go to original source... Go to PubMed...
    21. Patnaik AK, Hurvitz AI, Lieberman PH (1980): Canine hepatic neoplasms: A clinicopathologic study. Veterinary Pathology 17, 553-564. Go to original source... Go to PubMed...
    22. Patnaik AK, Hurvitz AI, Lieberman PH, Johnson GF (1981): Canine hepatocellular carcinoma. Veterinary Pathology 18, 427-438. Go to original source... Go to PubMed...
    23. Popp JA (1990): Tumors of the liver, gall bladder, and pancreas. In: Moulton JE (ed.): Tumors in Domestic Animals. University of California Press, Berkley. 436-445.
    24. Post G, Patnaik AK (1992): Nonhematopoietic hepatic neotologic features of well-differentiated hepatocellular car- plasms in cats: 21 cases (1983-1988). Journal of the Amercinoma in dogs. Veterinary Clinical Pathology 41, 382-390. ican Veterinary Medical Association 201, 1080-1082. Go to original source...
    25. Mizejewski GJ (1997): Alpha-fetoprotein as a biologic re- Ramaiah SK, Alleman AR (2002): Cytologic evaluation of sponse modifier: Relevance to domain and subdomain structure (minireview). Proceedings of the Society for Experimental Biology and Medicine 215, 333-362. Go to original source... Go to PubMed...
    26. Monlux AW, Anderson WA, Davis CL (1956): A survey of tumors occurring in cattle, sheep and swine. American Journal of Veterinary Research 17, 646-677.
    27. Mulligan RM (1949): Primary liver-cell carcinoma, hepatoma in the dog. Cancer Research 9, 76-81.
    28. Mulligan RM, Mulligan I (eds) (1949): Neoplasms of the dog. Williams and Wilkins, Baltimore. 111-114.
    29. Musahl C, Holthoff HP, Lesch R, Knippers R (1998): Stability of the replicative Mcm3 protein in proliferating and differentiating human cells. Experimental Cell Research 41, 260-264. Go to original source... Go to PubMed...
    30. Nakajima S, Doi R, Toyoda E, Tsuji S, Wada M, Koizumi M, Tulachan SS, Ito D, Kami K, Mori T, Kawaguchi Y, Fujimoto K, Hosotani R, Imamura M (2004): N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma. Clinical Cancer Research 10, 4125-4133. Go to original source... Go to PubMed...
    31. Nowak M, Madej JA (2006): Prevalence of neoplasms in domestic animals in Lower Silesia between 2000 and 2004 (in Polish). Medycyna Weterynaryjna 62, 900-904.
    32. Nowak M, Madej JA, Dziegiel P (2008): Correlation between the expression of Mcm-3 protein and Ki-67 antigen in mammary adenocarcinomas of dogs. Bulletin of the Veterinary Institute in Pulawy 52, 701-705.
    33. Nowak M, Madej JA, Ciaputa R, Poradowski D (2010): Manifestation of tumors in domestic animals in Lower the liver: aspiration findings and limitations. Compendium on Continuing Education for the Practising Veterinarian 24, 798-810.
    34. Remmele W, Stegner HE (1987): Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ERICA) in breast cancer tissue. Pathologie 8, 138-140.
    35. Schlageter M, Terracciano LM, D'Angelo S, Sorrentino P (2014): Histopathology of hepatocellular carcinoma. World Journal of Gastroenterology 20, 15955-15964. Go to original source... Go to PubMed...
    36. Song E, Reef E, Disler LJ, Kew MC (1982): Carcino-embryonic antigen in hepatocellular carcinoma. Report of a patient with very high serum concentrations. South African Medical Journal 62, 336-338.
    37. Teshima T, Matsumoto H, Shigihara K, Sawada H, Michishita M, Takahashi K, Koyama H (2013): Hepatocellular carcinoma in a young dog. The Canadian Veterinary Journal 54, 845-848. Go to original source... Go to PubMed...
    38. van Sprundel RG, van den Ingh TS, Guscetti F, Kershaw O, Kanemoto H, van Gils HM, Rothuizen J, Roskams T, Spee B (2013): Classification of primary hepatic tumours in the dog. The Veterinary Journal 19, 596-606. Go to original source... Go to PubMed...
    39. van Sprundel RG, van den Ingh TS, Guscetti F, Kershaw O, van Wolferen ME, Rothuizen J, Spee B (2014): Classification of primary hepatic tumours in the cat. The Veterinary Journal 202, 255-266. Go to original source... Go to PubMed...
    40. Withrow SJ (2001): Cancer of the gastrointestinal tract. Small Animal Clinical Oncology 3, 305-353. Silesia in 2005-2008. Bulletin of the Veterinary Institute Received: 2016-03-16 in Pulawy 54, 229-236.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content