Veterinární medicína, 2016 (vol. 61), issue 10

Antidermatophyte and antioxidant activities of Nigella sativa alone and in combination with enilconazole in treatment of dermatophytosis in cattleOriginal Paper

E. Balikci

Vet Med - Czech, 2016, 61(10):539-545 | DOI: 10.17221/32/2015-VETMED  

The purpose of this study was to comparatively assess the antidermatophyte and antioxidant activities of enilconazole, Nigella sativa (NS) and enilconazole with NS in the treatment of dermatophytosis in cattle. A total of 24 cattle with clinically established diagnosis of dermatophytosis were used in the study. Trichophyton verrucosum was isolated and identified from all of the specimens stemming from the dermatophytosis-suspected animals. The lesion areas in Groups 1, 2 and 3 were treated as follows: enilconazole (three times at 3-day intervals), NS (once a day for two weeks) and enilconazole with NS, respectively. There were significant...

Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogsOriginal Paper

R. Ciaputa, P. Bandoch, K. Lewandowska, J.A. Madej, M. Kandefer-Gola, I. Janus, M. Nowak

Vet Med - Czech, 2016, 61(10):546-552 | DOI: 10.17221/51/2016-VETMED  

In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies against carcino-embryonic antigen (CEA) cytokeratin (CK) 7 and 20, CD4, CD8, minichromosome maintenance protein 3 (MCM3), vimentin, and alpha 1 foetoprotein (AFP). In addition, Mallory's connective tissue stain, van Gieson's stain and Gomori methenamine silver stain were used. The study was performed on liver samples collected post mortem from five mixed-breed dogs aged 9-12 years. The tumours were classified according to the World Health Organization...

Changes in lymphocyte function and subset counts in cats with spontaneous chronic kidney diseaseOriginal Paper

S. Kralova-Kovarikova, L. Leva, Z. Knotek, M. Toman

Vet Med - Czech, 2016, 61(10):553-559 | DOI: 10.17221/282/2015-VETMED  

Chronic kidney disease alters the immune response in humans and dogs and it is thought to be one of the causes of immunosuppression in cats. Haematological, biochemical, and immunological examinations were performed on blood samples obtained from 20 cats with chronic kidney disease, and were compared with the results of 18 healthy cats. In cats with chronic kidney disease, statistically significantly lower numbers of total lymphocytes (P < 0.01), especially T lymphocytes (P < 0.001), were observed. When the results were compared with the lymphocyte transformation test in healthy cats, decreased responses to phytohaemaglutinin,...

Choice of staining technique affects the morphological assessment of epididymal feline spermOriginal Paper

C.C. Perez-Marin, E. Jimenez, E. Aguera

Vet Med - Czech, 2016, 61(10):560-566 | DOI: 10.17221/132/2015-VETMED  

Morphological examination of spermatozoa remains a valuable tool for determining the quality of feline sperm for cryopreservation and/or for use in artificial insemination. Numerous staining techniques have been employed for feline sperm assessment, and several variations have also been reported. Forty-eight domestic cats were castrated and epididymal sperm samples were obtained. Sperm morphology was assessed using two staining methods, an air-dried stain (Giemsa-Wright) and a wet stain (eosin-nigrosin), to determine their relative abilities for estimation of the proportion of normal and abnormal spermatozoa. The effects of centrifugation and the cooling...

Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit modelOriginal Paper

J. Vokurka, E. Gopfert, M. Blahutkova, E. Buchalova, M. Faldyna

Vet Med - Czech, 2016, 61(10):567-570 | DOI: 10.17221/24/2016-VETMED  

Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are platelet concentrates which have been used to improve healing and regeneration of damaged tissues. The concentration of growth factors greatly influences the final outcome of the treatment. For studying the effects of PRP and PRF, an animal model is required. The New Zealand White rabbit appears to be a good candidate for studying the features of these blood derivatives. This article presents for the first time the various preparation methods of blood derivatives with regard to concentration of growth factors - insulin-like growth factor, transforming growth factor and vascular endothelial...

Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccineOriginal Paper

L. Ondruska, V. Parkanyi, J. Vasicek, R. Jurcik, E. Hanusova, D. Vasicek, A. Balazi, F. Vizzarri

Vet Med - Czech, 2016, 61(10):571-576 | DOI: 10.17221/159/2015-VETMED  

The aim of this study was to evaluate the acute phase reaction and immune response of rabbits triggered by vaccination with a live attenuated myxoma virus (MXT) vaccine. Thirteen adult and 11 juvenile New Zealand white rabbit-based crossbreed rabbits, were used. Samples of rabbit peripheral blood were collected from vena auricularis centralis into heparinised tubes before vaccination and 48 h after vaccination. All animals were vaccinated by subcutaneous injection (0.5 ml) with a MXT vaccine. The blood plasma C-reactive protein level was measured by an ELISA kit using a double-antibody sandwich. For phenotyping of lymphocytes the fresh cells...

Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methodsOriginal Paper

A.J. Lora, A.M. Molina, C. Bellido, A. Blanco, J.G. Monterde, M.R. Moyano

Vet Med - Czech, 2016, 61(10):577-589 | DOI: 10.17221/212/2015-VETMED  

The purpose of this study was to evaluate the consequences of bisphenol A (BPA) exposure in zebrafish (Danio rerio). Short-term effects at a testes level were assessed by means of different biomarkers in 60 male zebrafish exposed for 14 days in aquariums to different concentrations of BPA (1, 10, 100 and 1000 µg/l) in addition to a control batch. The LC-MS/MS method was used for the quantification of BPA. The BPA levels in the zebrafish tissues increased together with increasing exposure of fish to BPA. Several alterations were observed in the zebrafish testes. Particularly notable morphological changes included, a pronounced degeneration...

Mesh repair of a large ventral hernia with interposition of omentum in a calf: a case reportCase Report

G. Giusto, C. Bellino, M. Casalone, V. Caramello, F. Comino, M. Gandini

Vet Med - Czech, 2016, 61(10):590-593 | DOI: 10.17221/272/2015-VETMED  

A one-month-old, Piedmontese female calf was admitted to the Department of Veterinary Sciences, University of Turin, for repair of a large ventral hernia. A large ventral hernia, approximately 20 cm long and 15 cm large was noticed extending from 3 cm caudal to the umbilicus down to the pubis. At ultrasonography the hernia content was represented by small intestine and omentum and no adhesions to the hernial sac could be detected. The hernial sac was composed by skin only. Because of the large dimensions of the defect and the economic value of the animal, surgical correction was recommended to the owner. A prosthetic implant with a polypropylene mesh...