Veterinární medicína - Latest articles
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Transitional, fused and/or supernumerary vertebrae in the lumbosacrocaudal region of the spine – A reality in many domestic mammals
C Toader Covasa
Vet Med - Czech, 2024, 69(5):156-168 | DOI: 10.17221/102/2023-VETMED 
The aim of this study was to identify the presence of transitional lumbosacral and sacrocaudal vertebrae in domestic mammals, to realise a comparative analysis of the localisation and conformation of this abnormal condition. The research included the following species: cattle – 29 specimens, sheep – 32 specimens, horse – 31 specimens, pig – 26 specimens, rabbit – 33 specimens, dog – 89 specimens and cat – 57 specimens. The spine of the animals was analysed post-mortem or radiologically. The investigations revealed the presence of transitional vertebrae as follows: in cattle – 3 cases (8.7%), all being...
Mammary neoplasms in female dogs: Clinical, diagnostic and therapeutic aspects
JR Rueda, CD Porto, RP Franco, IB da Costa, LMC Bueno, RJS Girio, FFR Manhoso, PCS Bueno, CSF Repetti
Vet Med - Czech, 2024, 69(4):99-114 | DOI: 10.17221/4/2024-VETMED
With the increase in the life expectancy of domestic animals and their increasingly affectionate relationship with their owners, it is possible to observe an increase in cases of neoplasms in these animals. Mammary neoplasia mainly affects older females who have not been castrated, due to hormonal dependence for the development of the tumour. The main form of treatment is surgery. This study aims to carry out an updated review on mammary neoplasms in female dogs covering the anatomy, physiology, prevalence, causes, diagnoses, treatments, prevention and prognosis, based on scientific articles by renowned researchers.
Repeated bolus injections of bupivacaine for continuous bilateral transversus thoracis plane block undergoing median sternotomy in a dog: A case report
D Kim, D Shin, S Yun, G An, J Jung, WG Son
Vet Med - Czech, 2024, 69(4):123-129 | DOI: 10.17221/118/2023-VETMED
An 8-year-old, 6.5 kg, neutered female Shih-Tzu dog was presented for surgical resection of a mediastinal mass. A median sternotomy and left cranial lung lobectomy were performed. Intraoperatively, with the patient under general anaesthesia, a bilateral transversus thoracis plane (TTP) block was performed by injecting 0.5% bupivacaine (0.2 ml/kg) per side using real-time ultrasound guidance. After surgery, indwelling catheters for repeated bolus injections of bupivacaine in TTP were placed as follows: the fifth sternebra was palpated in dorsal recumbency, and the transducer was placed in the longitudinal plane lateral to the sternal border. A 16 gauge...
Antibacterial effect of curcumin on Salmonella Typhimurium: In vitro and food model studies
G Terzi Gulel, S Kanat, E Kucukgoz
Vet Med - Czech, 2024, 69(4):115-122 | DOI: 10.17221/114/2023-VETMED
Salmonellosis is a major foodborne disease transmitted from contaminated poultry products worldwide. Although a wide variety of chemical agents are used in the prevention of foodborne Salmonella spp. infections, consumers prefer natural additives, that do not harm human health and do not impair the characteristics of food. Curcumin is a yellow-coloured, hydrophobic polyphenol obtained from the rhizome of the Curcuma longa L. plant known as turmeric. The purpose of this study was to evaluate curcumin’s antibacterial activity against S. Typhimurium in chicken meat and in vitro. In the first step, chicken samples...
Myxofibrosarcoma of the penis in an African pygmy hedgehog (Atelerix albiventris) – A clinical case
L Kasalova, H Cernochova, R Dvorakova, A Angelova, Z Knotek
Vet Med - Czech, 2024, 69(4):130-135 | DOI: 10.17221/107/2023-VETMED
A 3-year-old, 420 g, intact male African pygmy hedgehog (Atelerix albiventris) was presented with a sudden appearance of a mass protruding from its preputium. A detailed physical examination revealed the presence of a polyp-like mass, connected to the mucous membrane of the penis and a second, multilobular mass with a larger base. Both masses were surgically removed. While the histopathological examination of the polyp-like mass revealed only a chronic active inflammatory reaction, the histopathological examination of the multilobular mass revealed a tumorous tissue composed of spindle-shaped cells, irregularly oval or polygonal in some...
Assessment of deltamethrin-induced DNA damage, neurotoxic and neuroimmune effects in the brain tissue of brown trout (Salmo trutta fario)
T Karatas, M Cakir
Vet Med - Czech, 2024, 69(3):77-83 | DOI: 10.17221/115/2023-VETMED
This study investigated the impact of deltamethrin (DM) toxicity on brown trout (Salmo trutta fario), examining its effects on the immune system, including the white blood cell (WBC), lymphocyte (Lym), total immunoglobulin (T. Ig), and lysozyme levels, as well as its neurotoxic consequences on the brain tissue. The neurotoxic effects encompassed oxidative stress, the activity of the antioxidant enzymes, such as the superoxide dismutase (SOD) and catalase (CAT), acetylcholinesterase (AChE) activity, and DNA damage using 8-hydroxy-2-deoxyguanosine (8-OHdG). The DM exposure led to elevated levels of malondialdehyde (MDA), and 8-OHdG, while...
Effect of vitamin D3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid
A Gezer, S Yediel Aras, N Kilic Baygutalp, E Karadag Sari, G Bedir, B Mokhtare, K Yilmaz
Vet Med - Czech, 2024, 69(3):84-93 | DOI: 10.17221/111/2023-VETMED
In this study, the effects of vitamin D3 (Vit. D) and a stinging nettle (Urtica dioica L. (UD)) extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of CD. The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin...
Canine multiple primary tumours: Mammary tubular carcinoma, uterine leiomyosarcoma, and facial sebaceous epithelioma
Kim SH, Baek YB, SI Park
Vet Med - Czech, 2024, 69(3):94-98 | DOI: 10.17221/103/2023-VETMED
Multiple primary malignant tumours (MPMTs) are multiple neoplasms with independent pathogenetic origins, placing great importance on the tumorigenesis and clinical treatment. However, due to the rare occurrence and diagnostic confusion, MPMTs have rarely been investigated in veterinary medicine. In this report, a 10-year-old intact female Maltese dog had MPMTs, consisting of two malignant tumours and one benign tumour each derived from a topographically different site: tubular carcinoma in the mammary glands, leiomyosarcoma in the uterus and sebaceous epithelioma in the cheek. The unique combination of MPMTs would be the first case in veterinary...
Risk analysis and seroprevalence of bovine ephemeral fever virus in Punjab, Pakistan
S Nadeem, R Aslam, S Rahman, M Kasib Khan
Vet Med - Czech, 2024, 69(3):67-76 | DOI: 10.17221/95/2023-VETMED
Bovine ephemeral fever (BEF) is a vector-borne viral disease caused by the RNA virus which belongs to the genus Ephemerovirus and the family Rhabdoviridae. To evaluate the effect of the risk factors like the breed of cattle and buffaloes, age, sex, lactation, housing and region on the bovine ephemeral fever virus (BEFV) prevalence, ELISA and virus neutralisation (VN) tests (n = 600) were performed for the BEFV prevalence. The seroprevalence in cattle was 45.6% and 42% by ELISA and VN, respectively (P = 0.001). The breed-wise seropositive ratio was (55–64%) in cattle and (22.5–18.3%) in buffaloes by VN and ELISA....
Pharmacokinetics of praziquantel and its metabolites in grass carp (Ctenopharyngodon idella) following the oral administration of a single bolus
R Dobsikova, J Blahova, P Marsalek, V Doubkova, E Zuskova, J Velisek
Vet Med - Czech, 2024, 69(2):52-60 | DOI: 10.17221/109/2023-VETMED
The study aimed to evaluate and compare the routes and rates of the depletion of the antiparasitic praziquantel (PZQ), a derivative of pyrazinoisoquinoline, following its oral administration in grass carp (Ctenopharyngodon idella). We focused on the depletion of PZQ and its major metabolites – cis-hydroxy praziquantel (CPZQ) and trans-hydroxy praziquantel (TPZQ), in water, the plasma, hepatopancreas, kidney, muscle, and skin, following a single oral administration of PZQ in a concentration of 50 mg/kg. Fish were sampled before the drug administration and then eight times in the course of the 30-day-long experiment. Our results...
Concurrent primary splenic lymphoma and mammary gland tumour with polycystic ovaries in a dog
SH Kim, YB Baek, SI Park
Vet Med - Czech, 2024, 69(2):61-66 | DOI: 10.17221/99/2023-VETMED
Here, we report a rare case of concurrent primary splenic lymphoma and mammary gland tumour (MGT) with polycystic ovaries in a 10-year-old, intact female Jindo dog. The dog was presented with multiple masses in the fourth left mammary gland, the largest of which measured 6 cm in diameter, along with enlargement of the left inguinal lymph node on physical examination. Ultrasonography, radiography, and computed tomography scans revealed polycystic ovaries and a mass in the tail of the spleen, after total splenectomy and mastectomy with ovariohysterectomy, histopathological examination identified splenic diffuse large B cell lymphoma and malignant myoepithelioma...
Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province
DC Widianingrum, DG Silaban, WID Fanata, H Khasanah
Vet Med - Czech, 2024, 69(2):35-41 | DOI: 10.17221/80/2023-VETMED
Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in Escherichia coli (E. coli) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow’s milk and 71 goat’s milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT). E. coli was identified by phenotypic...
Evaluating the indicators of a heart rate variability analysis in dogs using Poincaré plots
T Kimura, S Shimamura, H Sakaya, T Hayashi, A Umemoto, K Sakai, M Yamasaki, T Hasegawa, T Shimada
Vet Med - Czech, 2024, 69(2):42-51 | DOI: 10.17221/49/2023-VETMED
Heart rate variability analyses using Poincaré plots can be useful for evaluating the autonomic nervous system function. However, the interpretation of the quantitative indicators of Poincaré plots remains controversial. Thus, few studies have verified the effectiveness of the quantitative indicators in veterinary medicine. This study aimed to verify the reliability of Poincaré plot indicators using pharmacological models in dogs. Four healthy beagles were used in this study. Each dog was treated with propranolol, atropine, and propranolol–atropine to block the sympathetic, parasympathetic, and sympathetic–parasympathetic functions, respectively....
List of volume 68 reviewers
Editorial Department
Vet Med - Czech, 2024, 69(1):I-II
Accidental salinomycin intoxication in European fallow deer (Dama dama L.)
M Svoboda, O Huml, P Chomat, A Honzlova, J Illek, Z Svobodova, L Hofmannova, H Modra
Vet Med - Czech, 2024, 69(1):18-29 | DOI: 10.17221/100/2023-VETMED
Salinomycin, belonging to ionophore antibiotics, has been used as a feed additive for poultry for its coccidiostatic effect. Poisoning by ionophore antibiotics has been reported in cattle and other sensitive animals due to the replacement of medicated feed and/or accidental overdoses. The aim of this paper is to report the toxicity of salinomycin for fallow deer and to describe the different levels of sensitivity of cervids to this substance. In the presented case study, a medicated feed containing ivermectin used for deworming red deer and fallow deer was accidentally contaminated with sodium salinomycinate in a concentration of 252.6 mg/kg....
In-feed Praziquantel treatment of grass carp (Ctenopharyngodon idella) infected with eye flukes
E Zuskova, J Velisek
Vet Med - Czech, 2024, 69(1):1-7 | DOI: 10.17221/97/2023-VETMED
Praziquantel (PZQ) is a drug effective against several parasitic diseases of fish caused by Platyhelminthes. The study assesses the efficacy of selected in-feed PZQ doses in eliminating metacercariae Diplostomum pseudospathaceum in grass carp. The fish were infected with D. pseudospathaceum in laboratory conditions and subsequently treated with PZQ at daily doses of 30 (PZQ1), 50 (PZQ2), and 100 (PZQ3) mg/kg bw for seven days. The treatment trial was followed by a 23-day observation period during which the fish were parasitologically examined on days 0, 2, 8 and 23. Smaller fish were infected with a significantly higher (P < 0.05)...
The effects of Pycnogenol, a pine bark extract on pulmonary inflammation by Asian sand dust in mice
SW Pak, SJ Lee, Kim WI, YG Yang, YK Cho, JS Kim, TW Kim, JW Ko, JC Kim, SH Kim, IS Shin
Vet Med - Czech, 2024, 69(1):8-17 | DOI: 10.17221/77/2023-VETMED
Erratum in: Vet Med-Czech. 2024 Nov 29;69(11):401. doi: 10.17221/97/2024-VETMEDAsian sand dust (ASD), also called China dust or yellow dust, mainly occurs in East Asia during spring and autumn. Because ASD enters the body mainly through the respiratory system, it can cause respiratory disorders or worsen underlying diseases. Because of this, it has become an important health concern that threatens the well-being of humans and animals. In this study, we investigated the effects of 15 and 30 mg/kg of Pycnogenol (PYC15 and 30 groups), a pine bark extract, on ASD-induced pulmonary inflammation in mice. We evaluated the inflammatory cell...
Double-wire woven nitinol stent for treating dogs with refractory tracheal collapse: A case series
JY Choi, Kim MY, HY Yoon
Vet Med - Czech, 2024, 69(1):30-34 | DOI: 10.17221/61/2023-VETMED
This retrospective case series describes the signalments, clinical signs, diagnostic test results, and postoperative complications obtained from the medical records of 10 client-owned dogs that underwent treatment for grade IV tracheal collapse using double-wire woven nitinol stents between October 2017 and September 2021. Respiratory signs resolved in all dogs immediately after tracheal stent placement. Mild to moderate stent fractures were identified in five dogs, of which two showed concurrent respiratory distress necessitating re-stenting after several months. Minor complications, such as stent migration, were absent. The double-wire woven...