Veterinární medicína, 2002 (vol. 47), issue 7

Molecular epidemiology of bovine tuberculosis in the Czech Republic and Slovakia in the period 1965-2001 studied by spoligotyping

I. Pavlik, L. Dvorska, M. Bartos, I. Parmova, I. Melicharek, A. Jesenska, M. Havelkova, M. Slosarek, I. Putova, G. Martin, W. Erler, K. Kremer, D. Van Soolingen

Vet Med - Czech, 2002, 47(7):181-194 | DOI: 10.17221/5822-VETMED  

Spoligotyping was used to examine IS6110-positive DNA of 26 Mycobacterium bovis, M. bovis BCG and M. bovis subsp. caprae non-viable isolates stored up to 10 years. All of these isolates were previously identified by biochemical tests and all 17/17 tested isolates were earlier found virulent for guinea pigs. In total seven spoligotypes, designated S1-S7, were detected and compared with the spoligotypes of 3 176 isolates in the database of the National Institute of Public Health and the Environment (RIVM) in Bilthoven, the Netherlands. A Neotype M. bovis strain, isolated in 1965 in the USA and thereafter...

Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams

M. Trávníček, D. Kováčová, M.R. Bhide, P. Zubrický, L. Čisláková

Vet Med - Czech, 2002, 47(7):195-198 | DOI: 10.17221/5823-VETMED  

Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF...

Clinical endocrinology of thyroid gland function in ruminants

Huszebucza GY, M. Kulcszr, P. Rudas

Vet Med - Czech, 2002, 47(7):199-210 | DOI: 10.17221/5824-VETMED  

After briefly introducing the basec steps in production and metabolism of thyroid hormones, the author gives an overview of nutritional, metabolic and disease related factors and endocrine interactions influencing thyroid gland function in ruminants, particularly in the postpartum dairy cow. Involvement of thyroid hormonal regulation of seasonal patterns of reproduction as well as ovarian endocrine function are discussed.

The Ecology of Mycobacteria

I. Pavlik, J. Kazda

Vet Med - Czech, 2002, 47(7):211-212 | DOI: 10.17221/5825-VETMED