Veterinární medicína, 2008 (vol. 53), issue 2
Systematic clinical examination of early postpartum cows and treatment of puerperal metritis did not have any beneficial effect on subsequent reproductive performance
R. Dolezel, M. Vecera, T. Palenik, S. Cech, M. Vyskocil
Vet Med - Czech, 2008, 53(2):59-69 | DOI: 10.17221/1988-VETMED 
The objective of this field trial was to evaluate the impact of a control program based on systematic clinical examination on Day 10 ± 3 post partum (pp) and treatment in the case of puerperal metritis on reproductive performance in dairy cows. Cows having serious dystocia as well as cows treated for retained placenta were not involved in the trial. The evaluation was performed by comparing reproductive performance between systematically examined cows (Group E, n = 83) and cows that were examined and treated occasionally on the basis of farmers' notifications of a pathological condition (Group C, n = 95). In Group E, reproductive...
Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro
M. Machatkova, P. Hulinska, J. Horakova, Z. Reckova, K. Hanzalova
Vet Med - Czech, 2008, 53(2):70-76 | DOI: 10.17221/1983-VETMED
The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5-9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1-5), middle (6-10) and late (11-14) luteal or early (Days 15-16), middle (17-19) and late (20-21) follicular phase. Oocytes were isolated separately from medium (5-9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least...
The effect of dietary linseed oils with different fatty acid pattern on the content of fatty acids in chicken meat
J. Zelenka, D. Schneiderova, E. Mrkvicova, P. Dolezal
Vet Med - Czech, 2008, 53(2):77-85 | DOI: 10.17221/1985-VETMED
Effects of 1, 3, 5 or 7% of linseed oil in the diet on the content of fatty acids in breast and thigh meat were studied in broiler chickens. Oils made either of seeds of the linseed cultivar Atalante (A) with a high content of α-linolenic acid or of the cultivar Lola (L) with a predominating content of linoleic acid were fed from 25 to 40 days of age. When feeding A, the contents of all n-3 polyunsaturated fatty acids (PUFA), including eicosatrienoic acid, were significantly higher, those of n-6 PUFA were lower, and the ratio of n-6/n-3 PUFA was narrower (P < 0.001) than when L was fed. The narrowest n-6 to n-3 PUFA ratio was observed...
Surveillance of viral fish diseases in the Czech Republic over the period January 1999 - December 2006
S. Reschova, D. Pokorova, J. Hulova, P. Kulich, T. Vesely
Vet Med - Czech, 2008, 53(2):86-92 | DOI: 10.17221/1982-VETMED
The present study reports the results of virological survey in fish with an emphasis on the diseases included in the Czech National Legislation. Monitoring of fish both without symptoms and with a manifested disease was performed within this virological surveillance. Between January 1999 and December 2006, viral haemorrhagic septicaemia virus (VHSV), infectious haematopoetic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) were surveyed by the National Reference Laboratory for viral diseases on fish. Among of 3789 sample pools processed, VHSV was detected in seven cases (6× in Oncorhynchus mykiss, 1× in Salmo trutta m....
Effects of postnatal exposure to benzo[a]pyrene on the immunity of immature rats
J. Matiasovic, L. Leva, J. Maskova, V. Kummer, M. Faldyna
Vet Med - Czech, 2008, 53(2):93-100 | DOI: 10.17221/1986-VETMED
To determine the effect of benzo[a]pyrene (B[a]P) on the developing immune system of immature rats, newborn animals received different concentrations (0.1, 1.0 and 10.0 mg/kg/day) of B[a]P in the first 14 days after birth. These rats were euthanised on day 23 of the experiment and it was found that the decreasing numbers of erythrocytes were in direct correlation to the dose of B[a]P. In subject animals, the overall counts of T cells in the spleen and the mitogenic activity increased. A decrease in the production of both IFN-γ and IL-4 (measured at the mRNA level) as well as the decreasing IFN-γ /IL-4 ratio in rat splenocytes were dependent...
Diagnostic testing of different stages of avian tuberculosis in naturally infected hens (Gallus domesticus) by the tuberculin skin and rapid agglutination tests, faecal and egg examinations
J.E. Shitaye, L. Matlova, A. Horvathova, M. Moravkova, L. Dvorska-Bartosova, I. Trcka, J. Lamka, F. Treml, V. Vrbas, I. Pavlik
Vet Med - Czech, 2008, 53(2):101-110 | DOI: 10.17221/1984-VETMED
Avian tuberculosis (ATBC) is a significant cause of morbidity and mortality in birds in zoos and breeding establishments. The primary sources of Mycobacterium avium subsp. avium (MAA) of serotypes 1, 2 and 3 are infected animals (esp. birds), who shed MAA in different ways and thus contaminate the environment. The first aim of this work was to compare the efficiency of the diagnostic methods that are routinely used for the diagnosis of ATBC (skin test, serology and culture of faeces and eggs) in naturally infected hens with different levels of infection. The second aim was to determine the excretion rate of MAA in faeces...
Lung tuberculosis in a horse caused by Mycobacterium avium subsp. avium of serotype 2: a case report
I. Pavlik, P. Jahn, M. Moravkova, L. Matlova, F. Treml, A. Cizek, E. Nesnalova, L. Dvorska-Bartosova, R. Halouzka
Vet Med - Czech, 2008, 53(2):111-116 | DOI: 10.17221/1987-VETMED
Interstitial pneumonia (2/3 of the lungs were affected) and diffusely enlarged bronchial and mediastinal lymph nodes were diagnosed by gross examination of a dead 16-year-old mare. Based on histopathological examination and the detection of acid-fast rods after staining by the Ziehl-Neelsen technique, tuberculosis was suspected. Mycobacterium avium subsp. avium of serotype 2 and IS901+/IS1245+ genotype was isolated from the pulmonary lymphnode after five-week incubation at 37°C. Due to the fact that horses have a naturally high resistance to mycobacterial infections, the high age of the mare most likely contributed to the...