Veterinární medicína, 2013 (vol. 58), issue 1

Structural homology and expression tendency of the natural immune response of the terminal complement components to inoculations in pigs: a reviewReview

D.V.A. Khoa

Vet Med - Czech, 2013, 58(1):1-9 | DOI: 10.17221/6650-VETMED  

The transmission of infectious agents from domestic animals to humans is a matter of particular concern at present. Inoculation can enhance the defences of each individual animal but only in the short term. Certainly, it will be of immense benefit if biotechnology and genetic techniques are applied to farm animal breeding and selection programs to improve productivity, performance and health status as well as for the construction of sustainable animal production systems and promotion of animal welfare. In recent years, efforts to drive candidate genes like cytokines, haptoglobin, complement system, C-reactive protein, a 2-macroglobulin, retinol binding...

The introduction of a double-channel system for the intrafollicular treatment of cattleOriginal Paper

S. Cech, J. Mala, E. Indrova, M. Lopatarova, R. Dolezel, H. Dluhosova, L. Zilka

Vet Med - Czech, 2013, 58(1):10-15 | DOI: 10.17221/6651-VETMED  

The aim of this study was to evaluate a new double-channel system for ultrasound-guided transvaginal intrafollicular treatment in cattle. The system is equipped with separate aspiration and treatment channels facilitating the aspiration of a small part of follicular fluid followed by the immediate injection of the same amount of treatment solution. In Experiment 1 an intracystic injection was performed successfully in all cows (6/6). In Experiment 2 saline was administered to dominant follicles with an overall success rate of 87.5% (14/16). This new double-channel system represents a reliable method for intrafollicular treatment preceded by an aspiration...

Morphological pattern of the livers of different lines of broiler chickens during rearingOriginal Paper

M. Gesek, J. Szarek, I. Otrocka-Domagala, I. Babinska, K. Pazdzior, M. Szweda, A. Andrzejewska, B. Szynaka

Vet Med - Czech, 2013, 58(1):16-24 | DOI: 10.17221/6652-VETMED  

The aim of this study was to analyse the microscopic and ultrastructural lesions of the livers of broiler chickens during fattening. Three genetic lines of broiler chickens (Ross 308, Cobb 500, Hubbard F15) were investigated. The liver samples were taken on the 3rd, 10th, 17th, 24th, 31st, and 38th day of life from six healthy broiler chickens from each commercial broiler flock. The dominant microscopic lesions were associated with prolonged hypoxia and bile ductules, including: fatty, vacuolar and parenchymatous degeneration, necrosis of epithelial cells of bile ductules, necrosis of...

Reduction of epidural fibrosis after laminectomy in rabbits by omental free graftOriginal Paper

N. Brkljaca Bottegaro, J. Kos, B. Pirkic, O. Smolec, Z. Grabarevic, M. Hohsteter, J. Selanec, Z. Vrbanac

Vet Med - Czech, 2013, 58(1):25-31 | DOI: 10.17221/6653-VETMED  

Epidural fibrosis is an extradural scar tissue formed after a laminectomy procedure. It is associated with persistent pain after spinal surgery and an increased risk of complications during revision surgery. The aim of this study was to determine the preventive effects of local application of an omental free graft in minimising spinal epidural fibrosis in a rabbit laminectomy model. Twenty two rabbits were randomly divided in two groups, a control group of seven and an experimental group of 15 animals. A dorsal laminectomy at levels L1 to L3 was performed on each rabbit of both groups. Prior to the laminectomy procedure, the animals...

A single nucleotide polymorphism in exon 3 of the myostatin gene in different breeds of domestic pigeon (Columba livia var. domestica) 32Original Paper

A. Dybus, W.S. Proskura, S. Sadkowski, E. Pawlina

Vet Med - Czech, 2013, 58(1):32-38 | DOI: 10.17221/6654-VETMED  

Myostatin is considered to be one of the most powerful negative regulators of muscle growth. The lack of a functional myostatin or its mutation can result in uncommon musculature like "double-muscling" in Belgian Blue cattle. Recent studies on MSTN variability indicate its relationship with racing performance in dogs and racehorses. Considering the high homology of MSTN among the vertebrates, there are grounds to suppose that the same correlation will occur in pigeons. The aim of this study was to analyse MSTN variability in several pigeon breeds raised for different purposes The PCR-RFLP method was used for genotyping the...

Detection of Anaplasma phagocytophilum in a catOriginal Paper

M. Gorna, L. Adaszek, K. Policht, M. Skrzypczak, S. Winiarczyk

Vet Med - Czech, 2013, 58(1):39-43 | DOI: 10.17221/6655-VETMED  

The aim of this study was to diagnose the etiological factor of disease in a 2.5 year old male cat with symptoms of loss of appetite, apathy and the presence of pale yellow mucous membranes. Haematological and biochemical analysis of the blood taken from the sick animal showed a normal white blood cell count (14.19 × 109/l), reduced erythrocyte (3.96 × 1012/l), haematocrit (0.2213), haemoglobin concentration (66 g/l) and thrombocyte count (52 × 109/l), increased activity of ALT (583 IU/l), AST (265 IU/l) and bilirubin concentration (205.2 µmol/l). A PCR test for Babesia/Theileria and Ehrlichia/Anaplasma...

Acute pancreatitis, azotaemia, cholestasis and haemolytic anaemia in a dog: a case reportCase Report

M. Guadarrama-Olhovich, L.E. Garcia Ortuno, J.A. Ruiz Remolina, C. Lopez Buitrago, J. Ramirez Lezama, J. Bouda

Vet Med - Czech, 2013, 58(1):44-49 | DOI: 10.17221/6657-VETMED  

We here report acute pancreatitis with multiorganic complications in a female Cocker Spaniel. The most important alterations in clinical pathology were renal azotaemia, hyperbilirubinaemia with a prevalence of conjugated bilirrubin and increased alkaline phosphatase by cholestasis; hyperamylasaemia due to pancreatitis; dehydration, hyponatraemia, hypochloraemia and hypokalaemia related to vomiting; metabolic acidosis and respiratory alkalosis corresponding to mixed acid-base disorder; markedly regenerative anaemia with spherocytes and agglutination due to immune-mediated haemolytic anaemia and intravascular haemolysis; leukocytosis with a left shift....

Augmentation of ruptured tendon using fresh frozen Achilles tendon allograft in two dogs: a case reportCase Report

M.R. Alam, W.J. Gordon, S.Y. Heo, K.C. Lee, N.S. Kim, M.S. Kim, H.B. Lee

Vet Med - Czech, 2013, 58(1):50-55 | DOI: 10.17221/6656-VETMED  

This article describes two cases of augmentation of ruptured tendon with fresh frozen Achilles tendon allograft (FFATA) in dogs. Case 1 was a two-year-old crossbreed dog (29 kg) that presented with an open wound on the right forelimb and with complete rupture of the flexor carpi ulnaris and superficial digital flexor tendons. Case 2 was a four-year-old crossbreed dog (4 kg) with partial ruptures of the patellar tendon and detachment of the tibial tuberosity in the right hind limb. In both cases, the ends of the ruptured tendon were sutured and apposed after debridement. To minimize suture failure, FFATA (cut to sufficient size) was placed...