Veterinární medicína, 2014 (vol. 59), issue 11
Electroretinography in dogs: a reviewReview
M. Drazek, M. Lew, S. Lew, A. Pomianowski
Vet Med - Czech, 2014, 59(11):515-526 | DOI: 10.17221/7816-VETMED 
Electroretinography (ERG) in the form of full-field, flash ERG is the most commonly used technique in veterinary ophthalmology for diagnosing the functioning of the outer retina. Under light stimulation spatially distributed different cell types within the retina produce time-varying electric responses. These are recorded in the form of ERG traces consisting of a series of positive and negative wavelets. The possibility of selective stimulation of individual types of retinal cells and the analysis of constituent components of ERGs are the basis for determining the source of abnormalities and diagnosis of various types of dysfunction. In many cases,...
Effect of medium chain fatty acids (MCFA) and probiotic (Enterococcus faecium) supplementation on the growth performance, digestibility and blood profiles in weanling pigsOriginal Paper
S. Mohana Devi, I.H. Kim
Vet Med - Czech, 2014, 59(11):527-535 | DOI: 10.17221/7817-VETMED
The objective of the present study was to evaluate the effects of MCFA and probiotic (Enterococcus faecium DSM 7134) supplementation on growth performance, nutrient digestibility, blood profiles, faecal score, excreta microbiota, and excreta noxious gas emission in weanling pigs. A total of 140 weanling pigs [(Yorkshire × Landrace) × Duroc] were allotted to four treatments groups of seven replicates/treatment and five pigs/replicate. The four experimental diets included: CON diet (basal diet); T1 (CON + MCFA 0.2%); T2 (CON + probiotic 0.01%) and T3 (CON + MCFA 0.2% + probiotic 0.01%). Growth performance, average daily gain (ADG), average daily...
Effects of summer shield supplementation on growth performance, nutrient utilisation, and plasma lipid profiles in broiler chickensOriginal Paper
A.A. Saleh, D. Ijiri, A. Ohtsuka
Vet Med - Czech, 2014, 59(11):536-542 | DOI: 10.17221/7818-VETMED
This study was conducted to evaluate the effects of herb mixture (summer shield) supplementation on growth performance, nutrient utilisation, and plasma lipid profiles in broiler chickens. Thirty-six male chicks at 15 days of age were divided into three groups with three replicates of four birds. The control group of chicks was fed a basal diet and the remaining two groups of chicks were fed the basal diet supplemented with summer shield at a concentration of 1 g/kg or 2 g/kg, respectively, until 37 days of age. Although feed intake was not different among the three groups, both body weight gain and breast muscle weight were increased in chicks fed...
The impact of pre-analytical treatment and sorting on human neutrophil functionOriginal Paper
L. Hromadkova, L. Zarybnicka, J. Vavrova, Z. Bilkova, Z. Sinkorova
Vet Med - Czech, 2014, 59(11):543-552 | DOI: 10.17221/7819-VETMED
This study was designed to test the hypothesis that pre-analytical treatment of relatively fragile cells is more important for their viability and function than shear forces and decompression shocks on a flow cytometric sorter. Human neutrophils were enriched using different techniques (sedimentation, hypotonic or ammonium chloride-mediated erythrocyte lysis) and their function was evaluated by measuring their capability of mounting oxidative burst. In contrast to other isolation techniques, neutrophils enriched by spontaneous sedimentation were found to be intact both in terms of their function and relative numbers within the leukocyte population;...
Effects of curcumin in combination with cyclophosphamide on canine mammary tumour cell linesOriginal Paper
F. Ustun Alkan, C. Anlas, S. Cinar, F. Yildirim, O. Ustuner, T. Bakirel, A. Gurel
Vet Med - Czech, 2014, 59(11):553-572 | DOI: 10.17221/7820-VETMED
In recent years, significant emphasis has been placed on combination chemotherapy in cancer using cytotoxic agents and plant derived-bioactive substances that are capable of selectively arresting cell growth and inducing apoptosis in tumour cells. The present study was undertaken to evaluate the possibility that the combination of curcumin and cyclophosphamide could show synergistic anti-proliferative effects towards CMT-U27 and CMT-U309 canine mammary cancer cells and, if so, to clarify the mechanism involved. The anti-proliferative activities of curcumin, cyclophosphamide and a combined treatment on CMT cells were determined using the MTT and LDH...
Fish kill caused by aluminium and iron contamination in a natural pond used for fish rearing: a case reportCase Report
A. Slaninova, J. Machova, Z. Svobodova
Vet Med - Czech, 2014, 59(11):573-581 | DOI: 10.17221/7821-VETMED
Contamination of Pansky Pond, in March 2013, with 119 mg/l aluminium, and 87 mg/l iron by acidic (pH 3.17) inflow from a nearby quarry caused fish die-off, while exhibiting symptoms of suffocation. Transformation of soluble forms of aluminium and iron into insoluble forms occurred on fish gill where the content of aluminium and iron was 100-fold and 12-fold, respectively, that found in control fish in an unaffected pond. In addition to insoluble aluminium and iron, gills showed presence of iron bacteria. Histopathology was characterised by expression of reactive processes and regressive alterations resulting in gill tissue necrosis. Impairment of the...