Veterinární medicína, 2015 (vol. 60), issue 5
Effects of season on plasma progesterone profiles in repeat breeding cowsOriginal Paper
M.E. Ghanem, M. Nishibori
Vet Med - Czech, 2015, 60(5):227-234 | DOI: 10.17221/8174-VETMED 
Forty six Holstein Friesian repeat breeding cows (the average AI/conception was 5.2 ± 0.2) were investigated using the progesterone assay after AI to determine possible differences in plasma progesterone profiles between summer and winter seasons. Twenty eight (60.9%) and 18 (39.1%) cows were followed in summer (June-August) and winter (December-February), respectively. In the summer season, the total progesterone concentrations were higher (P < 0.05) in pregnant cows with normal luteal function compared to those in non-pregnant animals with abnormal luteal function. In contrast, in the winter season, there was no difference (P...
Use of natural substances for boar semen decontaminationOriginal Paper
J. Mazurova, R. Kukla, M. Rozkot, A. Lustykova, E. Slehova, R. Sleha, J. Lipensky, L. Opletal
Vet Med - Czech, 2015, 60(5):235-247 | DOI: 10.17221/8175-VETMED
The aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected combinations of them against bacterial strains isolated from boar ejaculates was determined using the microdilution and macrodilution method in Mueller-Hinton broth. The most effective natural substances against Gram-negative and Gram-positive bacteria included in our study were thymol and carvacrol with minimum inhibitory concentration (MIC) values in the...
Evidence of Anaplasma spp. exposure in native Korean goats (Capra hircus coreanae)Original Paper
S.H. Lee, B.Y. Jung, D. Kwak
Vet Med - Czech, 2015, 60(5):248-252 | DOI: 10.17221/8176-VETMED
Anaplasmosis in animals is caused by Anaplasma spp. including A. phagocytophilum, A. marginale, A. centrale, A. ovis, and A. bovis, which are obligate intracellular rickettsial pathogens transmitted by ticks. Infection in animals is considered an important constraint on livestock production. In Korea, the prevalence of Anaplasma spp. has been investigated in several species, including cattle, dogs, and rodents, but there are no available data on anaplasmosis in goats. The purpose of this study was to investigate the presence of Anaplasma spp....
Serum luteinising hormone, testosterone and total cholesterol levels, libido and testicular histomorphology of male West African Dwarf goats orally or subcutaneously treated with monosodium l-glutamateOriginal Paper
I.S. Ochiogu, D. Ogwu, C.N. Uchendu, C.N. Okoye, J.I. Ihedioha, E.C. Mbegbu
Vet Med - Czech, 2015, 60(5):253-260 | DOI: 10.17221/8177-VETMED
This study investigated the effects of administration of monosodium l-glutamate (MSG) on serum luteinising hormone, testosterone and cholesterol levels, libido and testicular histomorphology of male West African Dwarf (WAD) goats. Thirty-two WAD goats (28 males and four females) were used for the study. The 28 males were randomly assigned to seven groups (A, BO, BS, CO, CS, DO and DS) of four goats each. Goats in groups BO, CO, and DO were given MSG orally at doses of 0.25, 0.50 and 1 g/kg body weight respectively for 28 days, while groups BS,...
Determination of the diversity and antibiotic resistance profiles of Staphylococcus species from dogs with otitis externa and examination of mecA gene occurrenceOriginal Paper
K. Metiner, A.F. Bagcigil, A. Ilgaz
Vet Med - Czech, 2015, 60(5):261-267 | DOI: 10.17221/8178-VETMED
The aim of this study was to determine the distribution of Staphylococci from swab samples of dogs with otitis externa and to determine their antibiotic resistance profiles, particularly methicillin resistance. For this purpose 116 ear swab samples were collected from 100 dogs and examined for the presence of Staphylococcus species by conventional culture methods. Antibiotic susceptibility of the isolates was determined by the disk diffusion test and for methicillin resistance, by PCR. Forty Staphylococci were isolated from 37 (31.9%) of the 116 ear swabs. Among the 40 isolates, 30 of them were coagulase-positive Staphylococcus species...
A simplified duplex real-time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae culturesOriginal Paper
S. Ehtisham-ul-Haque, S.U. Rahman, M.I. Khan, M. Younus, M.M. Awais, A. Nasir
Vet Med - Czech, 2015, 60(5):268-273 | DOI: 10.17221/8179-VETMED
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the present study was to develop a novel duplex TaqMan real-time PCR assay for the simultaneous detection of MG and MS. This duplex real-time PCR assay incorporates TaqMan (FAM/NED) labelled minor groove binder (MGB) probes that target the cytadhesin encoding surface protein (mgc2) gene and the haemagglutinin surface protein (vlhA) gene...
Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirusOriginal Paper
A. Kayar, B. Dokuzeylul, F.M. Kandemir, A. Kirbas, A. Bayrakal, M.E. Or
Vet Med - Czech, 2015, 60(5):274-281 | DOI: 10.17221/8180-VETMED
Feline coronavirus (FCoV) is a highly contagious virus that is ubiquitous in multicat environments and may induce oxidative stress. This virus commonly causes an asymptomatic infection, which can persist in certain individuals. Sporadically and unpredictably, FCoV infection leads to feline infectious peritonitis (FIP), a highly fatal systemic immune-mediated disease. There are no data in the veterinary literature relating to oxidative stress in FCoV. Antioxidant capacity (TAC) can be attributed to single components in the defence systems against free radicals. The measurement of the total oxidant status (TOS) accurately reflects the oxidative status...
Continuous ophthalmic treatment using an osmotic pump in a bull calf following surgical removal of an ocular dermoid: a case reportCase Report
J.H. Bae, C.E. Plummer, J. Kim, M.S. Kim, N.S. Kim
Vet Med - Czech, 2015, 60(5):282-287 | DOI: 10.17221/8181-VETMED
An intact male, six-month-old Hanwoo bull calf (native Korean beef breed) was presented to the Animal Medical Centre, Chonbuk National University because the owner had noticed a conjunctival and corneal abnormality in the left eye (OS). On ophthalmic examination, a small, elevated and skin-like mass lesion, containing hair was found on the ventronasal cornea and the conjunctiva of the third eyelid. In the light of its characteristic appearance, the lesion was classified clinically as a corneal dermoid. Under general anaesthesia, superficial lamellar keratectomy and conjunctivectomy was performed to remove the abnormal tissue. As the owner could not...
A cystoadenoma of the biliary ducts in a dog: anatomo-histopathological features and pathogenetic considerations: a case reportCase Report
B. Macri', G. Lanteri, M.T. Capucchio, A. Ieni, F. Marino
Vet Med - Czech, 2015, 60(5):288-291 | DOI: 10.17221/8182-VETMED
A rare case of a liver biliary duct cystoadenoma in a dog is reported. Tissue changes are described with an emphasis on immunohistochemical patterns that aid in the identification of the tumour and differentiation from similar macroscopical features that arise due to congenital abnormalities. Finally, we provide hints on comparative pathology.